Index Catalog // ScholarsArchive@OSU

Selection of an Orthogonal tRNA Synthetase for Trifluoromethyl-phenylalanine in Methanomethylophilus Alvus System

Creator:: Eyberg, Daniel R.
Abstract:: Genetic code expansion is a technology that allows for site-specific incorporation of noncanonical amino acids into recombinant proteins at amber stop codons using engineered translational machinery. The technology allows for expanded study into proteins, derived from the integration of novel chemical functions found in noncanonical amino acids, such as fluorescence,...
Resource Type:: Honors College Thesis
Full Text:: _____________________________________________________________________ Karen Van Zee, Committee Member, representing Biochemistry and Biophysics

Studies on the hydrophobic effect and its contribution to the stability, crystallization, and helix packing of Z-DNA

Creator:: Kagawa, Todd F.
Abstract:: The studies presented here use the B- to Z-DNA transition and Z-DNA crystallization as model systems to determine the contribution of solvent interactions to macromolecular structures. The substituent groups of naturally occurring and modified nucleotide bases affect the hydration and thus the stability of right-handed B-DNA and left-handed Z-DNA. The...
Resource Type:: Dissertation
Full Text:: determined by this interaction. The comparisons indicate that both solvent and van der Waals interactions

Controlling DNA packing in crystals

Creator:: Mooers, Blaine H. M.
Resource Type:: Dissertation
Full Text:: 1.3b & c). Favorable van der Waals interactions further stabilize these interduplex contacts. 10

The analysis and prediction of DNA structure

Creator:: Basham, Beth E.
Abstract:: As genome sequencing projects begin to come to completion, the challenge becomes one of determining how to understand the information contained within the DNA. DNA is a polymorphic macromolecule; the A- B- and Z-DNA conformations have been observed by a variety of physical techniques. The magnitude of the energetic differences...
Resource Type:: Dissertation
Full Text:: DNA solution structure and Dr. Kensal van Holde for his interest in this work. I also value the

Stabilization of Z-DNA by demethylation of thymine bases : a crystallization and thermo-dynamic study of d(m⁵CGUAm⁵CG)

Creator:: Zhou, Guangwen
Resource Type:: Dissertation
Full Text:: to express my appreciation to Dr. Kensal E. van Ho lde for his encouragement and to Dr. Christopher K

The asymmetric binding of Actinomycin D to DNA hexamers

Creator:: Ivancic, Monika
Abstract:: The solution structure determination of DNA molecules has been an important part of structural biology. NMR solution structures are a complement to structures solved via X-ray crystallography; the two methods are the only ways of obtaining three dimensional coordinates of macromolecules. Because of the nature of the molecule, the solution...
Resource Type:: Dissertation
Full Text:: spectroscopy is small compared with the number of solved protein structures (Wijmenga & van Buuren, 1998). Tn

The effect of cytosine methylation on DNA structure

Creator:: Vargason, Jeffrey M.
Abstract:: DNA methylation is common in prokaryotes and eukaryotes and has been implicated in various biological roles including gene silencing, X-chromosome inactivation, and genomic imprinting. 5-methylcytosine the "fifth base" of the genetic code comprises 1-3% of the human genome and is primarily found on cytosines within the context of the CpG...
Resource Type:: Dissertation
Full Text:: Acknowledgments We thank B. H. M. Mooers and the P. A. Karplus' laboratory for helpful discussion, and K. E. van

Vaccinia virus ribonucleotide reductase : gene sequencing, intracellular localization, and interaction with a DNA-binding protein

Creator:: Davis, Ralph Eugene, 1957-
Abstract:: Vaccinia virus infected monkey kidney cells had been previously shown to have an increased ribonucleoside diphosphate reductase (RR) activity. DNA from mutant virus resistant to hydroxyurea were digested with restriction endonucleases and were shown to have substoichiometric amounts of the Hind III F fragment. Additional information from Southern blotting experiments...
Resource Type:: Dissertation
Full Text:: of E. coli ribonucleotide reductase. (derived from Mathews and van Holde, 1990) Nucleotides Bound at

Vaccinia virus ribonucleotide reductase : regulation of the gene products and characterization of the recombinant small subunit protein

Creator:: Howell, Meredith L.
Abstract:: Ribonucleotide reductase is a remarkable enzyme that catalyzes the rate-limiting step in the synthesis of the 2'-deoxynucleoside triphosphates. The intent of this project was to characterize the ribonucleotide reductase encoded by the orthopoxvirus, vaccinia. The first objective was to study the structural and functional features of the viral small subunit...
Resource Type:: Dissertation

Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques

Creator:: Codding, Sara Jone
Abstract:: The focus of this dissertation was to understand protein function and structure ona molecular level. To do this successfully, a variety of biochemical and biophysicaltechniques were employed. A wide variety of techniques were applied to study theproteins of interest in this dissertation to probe protein-protein interactions, proteinfunction, protein structure, and...
Resource Type:: Dissertation

ScholarsArchive@OSU

Selection of an Orthogonal tRNA Synthetase for Trifluoromethyl-phenylalanine in Methanomethylophilus Alvus System

Studies on the hydrophobic effect and its contribution to the stability, crystallization, and helix packing of Z-DNA

Controlling DNA packing in crystals

The analysis and prediction of DNA structure

Stabilization of Z-DNA by demethylation of thymine bases : a crystallization and thermo-dynamic study of d(m⁵CGUAm⁵CG)

The asymmetric binding of Actinomycin D to DNA hexamers

The effect of cytosine methylation on DNA structure

Vaccinia virus ribonucleotide reductase : gene sequencing, intracellular localization, and interaction with a DNA-binding protein

Vaccinia virus ribonucleotide reductase : regulation of the gene products and characterization of the recombinant small subunit protein

Functional and Structural Analyses of Three Distinct proteins by Biochemical and Biophysical Techniques

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