Index Catalog // ScholarsArchive@OSU

Viral Targeting of Host Hubs: Interactions between the Rabies Virus and LC8

Creator:: Jespersen, Nathan E.
Abstract:: As obligate intracellular parasites, viruses rely on host machinery for their own reproduction. Viruses are therefore required to interact with a wide variety of host proteins, despite limitations in viral genome size. The most parsimonious method of success is to hijack central and essential proteins, known as hub proteins. LC8...
Resource Type:: Dissertation
Full Text:: AN ABSTRACT OF THE DISSERTATION OF Nathan E

Partial purification and characterization of the atrial muscarinic acetylcholine receptor

Creator:: Herron, G. Scott
Resource Type:: Dissertation
Full Text:: Muscarinic Acetylcholine Receptor AN ABSTRACT OF THE THESIS OF G. Scott Herron for the degree of Doctor of

Purification and characterization of an alkaline phosphatase from Halobacterium salinarium

Creator:: McParland, Reginald Harry
Abstract:: The halophilic alkaline phosphatase of Halobacterium salinarium has been purified to a specific activity of 3,000-3,200 units per mg of protein. The purification scheme consisted of acetone and ammonium sulfate fractionations, and molecular sieving and adsorption chromatography on Sephadex G -50. The enzyme was stabilized during purification by the presence...
Resource Type:: Dissertation
Full Text:: chromatography on Sephadex G -50. The enzyme was stabilized during purification by the presence of 0.01 M

Studies of rainbow trout Ki-ras gene : sequencing, aflatoxin B1 binding, and chromatin structure

Creator:: Liang, Xiaoshan
Abstract:: Characterization of the 5' flanking region of rainbow trout ki-ras gene was begun with the cloning and sequencing of this region by the inverse PCR technique and dideoxynucleotide chain termination method. In total, a nucleotide sequence of 1080 bp upstream from the first coding ATG was sequenced. Although this region...
Resource Type:: Dissertation
Full Text:: of codon 12 had differential affinity to AFB I, the more 5' G was relatively inaccessible but the

Studies on the growth inhibition and differentiation of serum-free mouse embryo (SFME) cells

Creator:: Varga Weisz, Patrick D.
Abstract:: Serum-free mouse embryo (SFME) cells are derived in medium in which serum is replaced with growth factors and other supplements. They display unusual properties. They do not lose proliferative potential or show gross chromosomal aberration upon extended culture, they depend on epidermal growth factor (EGF) for survival, and are reversibly...
Resource Type:: Dissertation
Full Text:: , bound to concanavalin A- agarose and was retarded by Sephadex G-50 in 200 mM acetic acid. A fifty to

The purification and characterization of the fructose-1, 6-diphosphate aldolase from Bacillus stearothermophilus (NCA 2184)

Creator:: Quinn, Richard Paul
Abstract:: The thermophilic aldolase from Bacillus stearothermophilus (NCA 2184) has been purified to a specific activity of 45.5 μ-moles of fructose-1, 6-diphosphate cleaved per minute per mg of enzyme, representing a 624-fold increase over the crude extract. The preparation appeared to be homogenous by sedimentation velocity ultra-centrifugation and by a specific...
Resource Type:: Dissertation
Full Text:: ultra- centrifugation and by a specific constant activity across the protein peak on Sephadex G-200

Site-specific aflatoxin B₁ adduction of sequence-positioned nucleosome core particles

Creator:: Moyer, Richard A.
Abstract:: The question of how the presence of nucleosomal packing of DNA modifies carcinogen interaction at specific sites cannot be answered by studies on whole chromatin or bulk nucleosomes because of the heterogeneity of DNA sequences in the particles. This problem was circumvented by constructing nucleosomes that are homogenous in DNA-histone...
Resource Type:: Dissertation
Full Text:: . MONONUCLEOSOME PREPARATION 24 E. PLASMID PREPARATION 25 F. PREPARATION OF END LABELED 5S DNA FRAGMENTS 28 G

Interaction of the human Voltage-Dependent Anion Channel 1 protein (VDAC-1) with Mycobacterium avium and its role in bacterial survival within phagocytic cells

Creator:: Pham, Tuan
Abstract:: M. avium is an opportunistic pathogen that primarily infects macrophages. In order to survive within the macrophage, M. avium secretes proteins into the host cell cytoplasm to inhibit specific functions such as phagosome acidification, altering pathways as well as initiating apoptosis. However, little is known about how those secreted proteins...
Resource Type:: Honors College Thesis
Full Text:: , and nitric oxide production (Nathan and Xie 1994), as well as the secretion of inflammatory

Characterization of photochemically cross-linked protein-nucleic acid complexes by mass spectrometry

Creator:: Jensen, Ole Norregaard
Abstract:: A novel protocol for the study of protein-nucleic acid interactions is presented and demonstrated to be feasible. The protocol combines photochemical crosslinking techniques and mass spectrometric methods into a new strategy for identifying protein domains or amino acid residues that are in close contact with nucleic acid in protein-nucleic acid...
Resource Type:: Dissertation
Full Text:: of Oregon) Mr. Stephen Swenson and Professor Steven Seifried (University of Hawaii) Mr. Samuel

Antisense Antibiotics

Creator:: Mitev, Georgi Mitko
Abstract:: Two different types of Phosphorodiamidate Morpholino Oligomers (PMOs) were used to inhibit growth in pure cultures of Salmonella enterica serovar typhimurium and in mouse macrophages infected with S. typhimurium. Both PMOs were 11 bases long and complementary to bases 6-16 of acyl carrier protein mRNA. A membrane-penetrating peptide, (RXR)4XB, was...
Resource Type:: Honors College Thesis
Full Text:: CFU/mL. The three hour culture was centrifuged at 5000 x g for 5 min. The supernatant was

ScholarsArchive@OSU

Viral Targeting of Host Hubs: Interactions between the Rabies Virus and LC8

Partial purification and characterization of the atrial muscarinic acetylcholine receptor

Purification and characterization of an alkaline phosphatase from Halobacterium salinarium

Studies of rainbow trout Ki-ras gene : sequencing, aflatoxin B1 binding, and chromatin structure

Studies on the growth inhibition and differentiation of serum-free mouse embryo (SFME) cells

The purification and characterization of the fructose-1, 6-diphosphate aldolase from Bacillus stearothermophilus (NCA 2184)

Site-specific aflatoxin B₁ adduction of sequence-positioned nucleosome core particles

Interaction of the human Voltage-Dependent Anion Channel 1 protein (VDAC-1) with Mycobacterium avium and its role in bacterial survival within phagocytic cells

Characterization of photochemically cross-linked protein-nucleic acid complexes by mass spectrometry

Antisense Antibiotics

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