Index Catalog // ScholarsArchive@OSU

The interaction of polylysine with DNA as studied by polarization of flurescence

Creator:: Evett, Jay Fredrick
Abstract:: A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl chloride (dansyl chloride), was covalently bonded to polylysine. The dansyl tagged polylysine was complexed to DNA by gradient dialysis. The polarization and intensity of fluorescence of the dansyl and the turbidity of the complex were measured as a function of salt concentration for a variety of...
Resource Type:: Dissertation
Full Text:: . Weber revived interest in the polarization of fluorescence method (59, 61) and utilized it to study the

Studies on the interaction of polylysine with deoxyribonucleic acid

Creator:: McKenzie, Raymond Leslie
Abstract:: The interaction of polylysine with DNA may be divided into two separate phenomena: the primary interaction involved in formation of a well-defined complex, and secondary interactions leading to aggregation of these complexes. Most studies on the interaction of polylysine with DNA have used techniques which measure properties of the aggregated...
Resource Type:: Dissertation
Full Text:: reported in the literature to be irre- versible at low ionic strength, i.e. 0.0 M to about 0.4-0.8 M NaCl

Nucleic acid polymerases associated with neoplasms

Creator:: Kiessling, Ann Drue
Abstract:: RNA tumor viruses, such as avian myeloblastosis virus (AMV) and MC29 virus, cause malignant transformation of the cells they infect. Transformed cells are able to continually produce progeny virus particles. Evidence is given that RNA-dependent RNA polymerase is present in virus infected cells. RNA-dependent RNA polymerase isolated from AMV-infected myeloblasts...
Resource Type:: Dissertation
Full Text:: investigations. Appreciation is given to Dr. Anne O'C. Deeney and George H. Weber for making available the

Fatty acids in the eggs and sac fry of steelhead trout (Salmo Gairdneri) : a developmental study

Creator:: Hayes, Lyle Wayne
Abstract:: Developing steelhead trout eggs and sac fry were examined for wet, dry and lipid weights and for fatty acid composition over the period from fertilization until the disappearance of the yolk sac, 69 days after fertilization. The yolk sac and embryo or fry were separated shortly before hatching, and the...
Resource Type:: Dissertation
Full Text:: accelerating phase of embryonic growth, can often be fitted with an equation of the form M = Kot k 1, where M

Monophoton decay time fluorometry : theory, design, and application to biochemical systems

Creator:: Schuyler, Robert John
Abstract:: In the determination of binding heterogeneity and rotational diffusion relaxation times, fluorescence decay curves often take the form of sums of exponential decay times. An instrument is described which includes features permitting the collection of high precision time decay data, which may successfully be resolved into component decays. The instrument...
Resource Type:: Dissertation
Full Text:: introduction by Weber in 1952. Information about the rotatory motions and sizes of proteins and nucleic acids

Glutamate dehydrogenase : I.Flourescence polarization studies of the self-association. II.Flourescence and circular dichroism studies of the heterotropic interactions

Creator:: Malencik, Dean Anthony
Abstract:: Fluorescence polarization measurements on beef liver glutamate dehydrogenase conjugated with pyrene butyric acid demonstrate that the association of the enzyme is adequately described by a reversible indefinite association with a single equilibrium constant. The data obtained with protein concentrations up to 0.7 mg/ml in 0.05 M potassium phosphate, pH 7....
Resource Type:: Dissertation
Full Text:: protein concentrations up to 0.7 mg/ml in 0.05 M potassium phosphate, pH 7. 6, are consistent with a

The effect of coenzyme on the bis(1-anilino-8-naphthalenesulfonate) induced association of lactic dehydrogenase

Creator:: Lu, Fung-jou
Resource Type:: Masters Thesis
Full Text:: Gregorio Weber. The dye was dissolved in glass-distilled water to give 10 -3 M stock solutions. 5 An

Subunit structure of Cancer magister Hemocyanin

Creator:: Carpenter, Donald Ellis
Resource Type:: Dissertation
Full Text:: filtration of reduced and alkylated hemocyanin on a 6 M GuHC1 - 6% agarose column gave only one peak. The

Conformational changes in histone IV as induced by salts and nucleoside triphosphates

Creator:: Wickett, Richard Randall
Abstract:: The addition of salts or nucleoside triphosphates to histone IV solutions induces conformational changes in the histone molecule. These conformational changes are investigated by measurement of the fluorescence anisotropy of the histone tyrosine emission, the circular dichroism (CD) of the peptide bond and of the tyrosine CD. Changes induced by...
Resource Type:: Dissertation
Full Text:: histone IV in water, in 3.3 x 10 3 M phosphate, pH 7.4 recorded from t = 25 to 55 min, from t = 290 59 320

The influence of monovalent cations on tryptophanase and pyruvate kinase as studied by fluorescence, circular dichroism, and kinetic methods

Creator:: Leete, John Huntington
Abstract:: Two spectrally different species of holotryptophanase were obtained from fluorescence and circular dichroism measurements. One is predominant in solutions containing certain enzyme activating monovalent cations while the other predominates in solutions of various inhibiting monovalent cations. Relative magnitudes of the cation sensitive fluorescent intensity maxima or circular dichroism maxima are...
Resource Type:: Dissertation
Full Text:: possible binding site model for ICE in holo- tryptophanase. 37 12. A Hill plot of the effect of 0.11 M Na

ScholarsArchive@OSU

The interaction of polylysine with DNA as studied by polarization of flurescence

Studies on the interaction of polylysine with deoxyribonucleic acid

Nucleic acid polymerases associated with neoplasms

Fatty acids in the eggs and sac fry of steelhead trout (Salmo Gairdneri) : a developmental study

Monophoton decay time fluorometry : theory, design, and application to biochemical systems

Glutamate dehydrogenase : I.Flourescence polarization studies of the self-association. II.Flourescence and circular dichroism studies of the heterotropic interactions

The effect of coenzyme on the bis(1-anilino-8-naphthalenesulfonate) induced association of lactic dehydrogenase

Subunit structure of Cancer magister Hemocyanin

Conformational changes in histone IV as induced by salts and nucleoside triphosphates

The influence of monovalent cations on tryptophanase and pyruvate kinase as studied by fluorescence, circular dichroism, and kinetic methods

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