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Filtering by: Creator Wong, George Kaon Remove constraint Creator: Wong, George Kaon Degree Field Biochemistry and Biophysics Remove constraint Degree Field: Biochemistry and Biophysics

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  • Analysis of ras gene mutations in rainbow trout tumors

    Creator:
    Chang, Yung-jin
    Abstract:
    For ras gene mutation analysis in the rainbow trout (Oncorhynchus mykiss) model system, a partial trout ras sequence was identified using the polymerase chain reaction (PCR). Two synthetic oligonucleotides based on rat K-ras gene sequence were used as primers for the PCR procedure. A 90 base pair (bp) sequence, referred...
    Resource Type:
    Dissertation
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    Tumors. Abstract approved : Redacted for Privacy George S. Bailey For ras gene mutation analysis in

  • Site-specific aflatoxin B₁ adduction of sequence-positioned nucleosome core particles

    Creator:
    Moyer, Richard A.
    Abstract:
    The question of how the presence of nucleosomal packing of DNA modifies carcinogen interaction at specific sites cannot be answered by studies on whole chromatin or bulk nucleosomes because of the heterogeneity of DNA sequences in the particles. This problem was circumvented by constructing nucleosomes that are homogenous in DNA-histone...
    Resource Type:
    Dissertation
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    privacy George T. Bailey The question of how the presence of nucleosomal packing of DNA modifies

  • Sequence conversion during adenovirus DNA replication

    Creator:
    Bennett, Kelly L.
    Abstract:
    The work in this thesis has provided conclusive genetic evidence that "panhandle" intermediates form during adenovirus replication. Adenovirus chromosomes lacking 51 by from their left -hand termini are infectious and capable of regenerating the missing origin sequence. Yet if an entire inverted terminal repeat is removed, the adenovirus chromosome is...
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    Dissertation
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    . George Pearson. The support he provided for me is greatly appreciated. In addition, I would like to

  • Characterization of neuronal nitric-oxide synthase reductase activity

    Creator:
    Wolthers, Kirsten R.
    Abstract:
    During catalysis the flavoprotein domain of neuronal nitric-oxide synthase (nNOS) shuttles NADPH-derived reducing equivalents from FAD to FMN and then to the P450-heme enabling heme-based oxygen activation and subsequent NO-synthesis. The binding of Ca²⁺-activated calmodulin (Ca²⁺-CaM) to nNOS alleviates inhibition of flavin mediated electron transfer within the diflavin domain, which...
    Resource Type:
    Dissertation
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    of my committee, Drs. Sonia Anderson, Dale Mosbaugh, Stephen Giovannoni, and Isaac Wong. I am also

  • Studies of rainbow trout Ki-ras gene : sequencing, aflatoxin B1 binding, and chromatin structure

    Creator:
    Liang, Xiaoshan
    Abstract:
    Characterization of the 5' flanking region of rainbow trout ki-ras gene was begun with the cloning and sequencing of this region by the inverse PCR technique and dideoxynucleotide chain termination method. In total, a nucleotide sequence of 1080 bp upstream from the first coding ATG was sequenced. Although this region...
    Resource Type:
    Dissertation
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    , encouragement, and financial support; especially for providing me a chance to work on this project. Dr. George

  • Site-directed mutagenesis of the m2 muscarinic acetylcholine receptor

    Creator:
    Vogel, Walter Kevin
    Resource Type:
    Dissertation
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    thank Christopher K. Mathews, George J. Mpitsos, Thomas F. Murray, and Kensal E. van Holde for serving

  • The interaction of polylysine with DNA as studied by polarization of flurescence

    Creator:
    Evett, Jay Fredrick
    Abstract:
    A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl chloride (dansyl chloride), was covalently bonded to polylysine. The dansyl tagged polylysine was complexed to DNA by gradient dialysis. The polarization and intensity of fluorescence of the dansyl and the turbidity of the complex were measured as a function of salt concentration for a variety of...
    Resource Type:
    Dissertation
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    , ribonuclease, DNA, and myosin is taken from von Hippel and Wong (56). The effect of ions on the binding of

  • Model systems for structural investigations into peroxiredoxin catalysis, conformation change, and inactivation

    Creator:
    Perkins, Arden
    Abstract:
    Peroxiredoxin (Prx) enzymes catalyze the reduction of hydrogen peroxide, peroxynitrous acid, and organic peroxides, and are extremely efficient peroxidases, with k[subscript cat]/K[subscript M] on the order of 10⁷ - 10⁸ M⁻¹ s⁻¹. Besides their role in oxidative stress defense, evidence has accumulated that some eukaryotes, including humans, use Prxs as...
    Resource Type:
    Dissertation

  • Interaction of linker proteins, H1 and HMG1, with nucleosome reconstituted on positioning sequences

    Creator:
    An, Woojin
    Abstract:
    The aim of this research was to gain more understanding on how a linker histone (LH), H1⁰, and high mobility group protein, HMG1, interact with nucleosome. To determine the location of linker proteins, three specific positioning sequences were used to reconstitute core nucleosomes and the binding of H1 and HMG1...
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    Dissertation
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    people I worked with for helping me when I was in difficulties: Tom Allan, Dr. George Carter, Ahmed

  • Nucleoprotein structure of adenovirus

    Creator:
    Corden, Jeffry Lynn
    Abstract:
    The structure of the nucleoprotein core of adenovirus has been investigated. Using a combination of biophysical techniques and nuclease digestion I have determined that the adenovirus core, as isolated with pyridine, shares several physical properties with cellular chromatin. The most notable difference is the lack of a nucleosome repeat in...
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    Dissertation
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    ACKNOWLEDGMENTS I thank Professors George Pearson and Ken Van Ho lde for advice, encouragement, and support