Three types of experiments were designed to further characterize
the tryptophan decarboxylating enzyme of Reed and Crecelius
(1967).
1) Light inhibition studies were made with etiolated pea seedlings
to determine the in vitro response of the tryptophan decarboxylating
enzyme. Assays of indoleacetic acid oxidase (IAA oxidase)
and guaiacol-active pea seedling...
The rate of the C¹⁴0₂ release from glucose, specifically labeled
with C¹⁴ at 1, 2, 3+4, and positions, was used to study the relative
participation of pathways of glucose catabolism in red beet slices.
Evidence was obtained which indicated that in mature red beets glucose
was catabolized mainly via glycolysis-tricarboxylic...
Partial purification of diamine oxidase from pea seedlings,
Pisum sativum, was accomplished by homogenization of 8-10 day
etiolated epicotyl tissue, followed by ammonium sulfate fractionation
and DEAE Sephadex column chromatography. The preparation thus
obtained was purified 50 fold.
Some properties of this enzyme were investigated. A radio-tracer method was adapted...
E. coli thioredoxin (TRX) was modified by the episulfonium ion derived from S-(2-chloroethyl)glutathione (CEG) or S-(2-chloroethyl)cysteine (CEC). The alkylation site was located at Cys-32, which was confirmed by tandem mass spectrometry. Two forms of native TRX, Oxi- and Red-TRX, and two modified TRXs, GS- and Cys-TRX, were examined by hydrogen/deuterium...
The investigation
of impurity effects on the properties of alkali
halide crystals has often been difficult because of the variation of procedures used in various experiments.
The purpose of this work is to
develop a self consistent method of measuring the IV dipole polarization properties of single crystals while eliminating...