The rate of the C¹⁴0₂ release from glucose, specifically labeled
with C¹⁴ at 1, 2, 3+4, and positions, was used to study the relative
participation of pathways of glucose catabolism in red beet slices.
Evidence was obtained which indicated that in mature red beets glucose
was catabolized mainly via glycolysis-tricarboxylic...
Three types of experiments were designed to further characterize
the tryptophan decarboxylating enzyme of Reed and Crecelius
(1967).
1) Light inhibition studies were made with etiolated pea seedlings
to determine the in vitro response of the tryptophan decarboxylating
enzyme. Assays of indoleacetic acid oxidase (IAA oxidase)
and guaiacol-active pea seedling...
Partial purification of diamine oxidase from pea seedlings,
Pisum sativum, was accomplished by homogenization of 8-10 day
etiolated epicotyl tissue, followed by ammonium sulfate fractionation
and DEAE Sephadex column chromatography. The preparation thus
obtained was purified 50 fold.
Some properties of this enzyme were investigated. A radio-tracer method was adapted...
E. coli thioredoxin (TRX) was modified by the episulfonium ion derived from S-(2-chloroethyl)glutathione (CEG) or S-(2-chloroethyl)cysteine (CEC). The alkylation site was located at Cys-32, which was confirmed by tandem mass spectrometry. Two forms of native TRX, Oxi- and Red-TRX, and two modified TRXs, GS- and Cys-TRX, were examined by hydrogen/deuterium...
In this dissertation, a novel method for analyzing computed transition states (TS) for organic reactions is disclosed. It introduces the use of localization-delocalization matrices as parameters in a linear free energy relationship to determine the transition state interactions responsible for differences in energy between the major and minor TS in...
An attempt has been made to elucidate the biosynthetic precursor
of ethylene in the mold P. digitatum. By application of basic
radiotracer methodology and the utilization of several specifically
labeled radiochemical substrates, it has been possible to demonstrate
that the internal carbon atoms of C-4 dicarboxylic acids such
as succinate,...
A long path dc plasma arc operated in an atmosphere of
argon is described. The arc is easily ignited and operated, and
is inexpensive to use. Samples were introduced into the arc as
solution aerosols. Radially resolved emission measurements are
reported for calcium, copper, and hafnium. The detection limit
for...
Little is known regarding the catabolic mechanisms involved
in the breakdown of glucose in mung bean (Phaseolus aureus)
seedlings. Studies in other laboratories have shown that the TCA
cycle pathway is operative in mung bean leaves, and have demonstrated
the presence of enzymes for the oxidation of glucose to
glucuronic...