Cellulolytic enzymes capable of efficiently degrading crystalline cellulose are a
complex mixture of endo- (endoglucanases) and exo-acting (cellobiohydrolases)
enzymes. One approach to separating these enzymes is affinity chromatography. A new
ligand, p-aminophenyl l-thio-β-D-cellobioside (APTC), is introduced for this purpose.
The property of APTC in affinity chromatography is demonstrated using Trichoderma...
Hazelnuts are commonly consumed raw and are valued for their numerous health benefits and antioxidant properties. Increased foodborne illness outbreaks associated with Salmonella and Escherichia coli O157:H7 contamination of tree nuts and peanuts generate a need for improving agricultural sanitation procedures. Food-safe chemical sanitizers have shown promise for reducing pathogenic...
Pectinmethylesterase (PME) activity in fresh cucumber
fruit was determined. The effects of several cationic
species, temperature, pH, and acetic acid on the activity
of cucumber PME was investigated. The efficacy of
blanching, infiltration with CaCl₂ or acetic acid, and
fermentation with 0 and 40 mM CaCl₂ on inhibition of
cucumber...
Intrinsic characteristics of Pacific sardines were determined. The lipid content
in sardines was initially low (6.79%) in the beginning ofthe season, increased to
22.95% in mid-August, and decreased slightly by the end of September. An inverse
correlation (R² = -0.90) was found between lipid and moisture content. Analysis
showed that...
The objectives of this study are to 1) qualitatively and quantitatively measure the odor-active compounds in ‘Marion’ blackberry; 2) compare the aroma profiles of advanced selections and new cultivars of thornless blackberry cultivars with ‘Marion’; 3) study the development of furaneol in ‘Marion’ during ripening and; 4) investigate the distribution...
Blackberries have been enjoyed for their delicious flavor and high nutritional value for hundreds of years. In North America, blackberries were cultivated since 1850-1860. Blackberries have their characteristic and unique aromas depending on the cultivar and growing conditions. The objective of this study was to evaluate the impact of seasonal...
Grapevine red blotch virus (GRBV) is a single-stranded DNA virus that causes grapevine red blotch disease (GRBD). Symptoms of GRBD include red discoloring of leaf margins and red veins on the underside of the leaves. GRBD affects the plant's metabolism and photosynthetic pathways, and disrupts carbon translocation and chlorophyll concentrations....
Cheddar Cheese samples (good cheese, weak cheese, cheese made with pasteurized milk, cheese made with heat-shocked milk, cheese from production plant A, cheese from production plant B, cheese made with adjunct culture, and cheese made without adjunct culture), were evaluated during the ripening stage. Proteolysis was studied by a fractionation...
A low, but significant, fraction of the carbohydrate portion of herbaceous biomass may be composed of fructose/fructosyl-containing components (“fructose equivalents”); such carbohydrates include sucrose, fructo-oligosaccharides, and fructans. Standard methods used for the quantification of structural-carbohydrate-derived neutral monosaccharide-equivalents in biomass are not particularly well suited for the quantification of fructose equivalents...
Three analytical methods for determination of aroma compounds in alcoholic beverages were developed and validated. These methods are sensitive and reliable that can be applied to analyze volatile phenols, stale aldehydes as well as some highly volatile aromas in alcoholic beverages. In the first study, an ethylene glycol (EG) polydimethylsiloxane...