To help understand the mechanisms of conifer chloroplast genome evolution, we characterized the nucleotide sequences of Douglas-fir (Pseudotsuga menziesiz) and
Monterey pine (Pinus radiata) chioroplast DNA that are associated with a length
mutation hotspot and inversion. The chloroplast DNA ofthese two conifers are
characterized by shared large inversions that are...
A crucial factor in determining the accuracy of DNA replication is
maintenance of a balanced supply of deoxyribonucleoside triphosphates
(dNTPs) at replication forks. Perturbation of dNTP biosynthesis can
induce dNTP pool imbalance with deleterious genetic consequences,
including increased mutagenesis, recombination, chromosomal
abnormalities and cell death. Using the T4 bacteriophage system,...
We studied genetic polymorphism and phylogeny using nuclear random amplified polymorphic DNA markers (RAPDs) and mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) in the three California Closed-Cone Pines: Pinus attenuata Lemm., P. muricata D. Don, and P. radiata D. Don. A total of 343 to 384 trees derived from...
Chloroplast genomes of conifers are unusual in a
number of respects, including the possession of much
repetitive DNA. By studying the distribution and sequences
of dispersed repetitive DNA, I hoped to gain insight into
the mechanisms of chloroplast genome evolution.
I used restriction mapping and DNA sequencing to
characterize dispersed...
A gene cluster responsible for the biosynthesis of the antitumor agent
cetoniacytone A was identified in Actinomyces sp. strain Lu 9419, an
endosymbiotic bacteria isolated from the intestines of the rose chafer beetle. The
nucleotide sequence analysis of the 26 Kb DNA region revealed the presence of 17
complete ORFs,...
Influenza A and Respiratory Syncytial Virus (RSV) are both enveloped, negative strand
RNA viruses which infect the respiratory mucosa of animals and humans. Despite
decades of research and development of antivirals and vaccines, both of these viruses
continue to be a major health concern throughout the world. The focus of...