The species composition of soil microfungal populations in-adjacent
stands of red alder, conifers, and mixed alder conifer correlated
strongly with the dominant vascular vegetation. A total of 92
species were isolated: 55 from the alder stand; 45 from the conifers;
and 46 from the mixed alder-conifer, with few species (16,...
The virion protein patterns of 72 isolates of infectious
hematopoietic necrosis virus (IHNV) were analyzed by sodium dodecyl
sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of S-methionine
labeled virus. This analysis led to the classification of these virus
isolates into 4 or more types. Type 1 virus is characterized by a
nucleocapsid protein...
The role of viral proteins in the pathogenesis of infectious hematopoietic necrosis
virus (IHNV) was studied at the molecular level. The expression of the viral genes at the
protein and RNA level, and their cellular localization, were characterized to further our
understanding of viral pathogenesis. The pathogenic effect of individual...
Disc gel electrophoresis of the vegatative cell -free extracts ofstrains of Clostridium botulinum types A, B, C, E, and F and therelated nontoxic group showed limited value as a means for identifyingthese closely related microorganisms, since separation,though not consistent in all cases, could only be based on the numberof protein...
A water-soluble complex containing ergosterol together with
a component of yeast has been isolated. The complex can be isolated
from commercial yeast extract to which ergosterol has been
added or directly from whole yeast cells. The complexing agent
from yeast extract is also capable of solubilizing cholesterol and
a long...
From a population of cells of Pseudomonas aeruginosa sensitive 33 parts per million (ppm) and 0.1 mg per ml of quaternary
ammonium (QAC) and chloramphenicol respectively, pure strains
resistant to 750 ppm and 50 mg per ml of these antibiotics were isolated.
Lipids from the sensitive and resistant cells grown...
Studies were directed toward the formulation of a selective
Aeromonas medium that would permit the isolation of Aeromonas
species in the presence of other common bacterial flora. The final
composition of this medium, PBG Agar, in grams per liter, is:
Bacto-peptone, 10; Bacto-beef extract, 10; glycogen, 4; NaCl, 5;
sodium...
The immune systems of various teleost fish have been studied in some detail for
the past several decades. One aspect of fish immunity, that of endogenously produced
modulating factors, has recently received a great deal of attention. Understanding the
functions and roles of endogenous factors that regulate fish immunity is...
A method for rapid isolation of plasmid DNA from group N
streptococci was developed. Chief advantages of the method were
simplicity, the utilization of microliter quantities of reagents,
and the obtainment of preparations highly enriched for covalently
closed circular plasmid DNA. The method was also applied to over
15 Leuconostoc...
The regulation of ergosterol biosynthesis in yeast has been
studied. The system has been examined for the presence of an end-product inhibition. Both anaerobically and aerobically grown cells
have been examined for this effect. A feedback inhibition of sterol
synthesis has been shown, but it appears to be a secondary...
Salmonid B lymphocytes were stimulated both in vivo and in vitro
and shown to produce antibody specific for the immunogen. A passive
hemolytic plaque assay was employed to demonstrate B cells producing
specific antibody. Characterization of plaque forming cells (PFCs)
involved sequential inhibition with a series of graded concentrations
of...
Unfertilized ova from spring chinook salmon (Oncorhynchus
tshawytscha) were examined for the presence of classical salmonid
inmmunoglobulin. Immunodiffusion techniques in which rabbit anti-salmonid
immunoglobulin was reacted against an ova homogenate
and rabbit anti-ova homogenate was reacted against immune chinook
serum failed to detect classical-type antibodies within these eggs.
A lectin...
Fish diseases and various parameters associated with disease
caused mortality of fish were monitored at the Oregon State University
Marine Science Center and at a private mariculture facility on
Yaquina Bay during a period of five years. Nearly all disease problems
observed were caused by Vibrio anguillarum and Vibrio spp....