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Filtering by: Advisor Hawkes, Stephen J. Remove constraint Advisor: Hawkes, Stephen J. Degree Field Microbiology Remove constraint Degree Field: Microbiology License All rights reserved Remove constraint License: All rights reserved

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  • Quantification of Osmolytes in the Sargasso Sea Surface Layer Water Column

    Creator:
    Muslin, Omran
    Abstract:
    Oceanic carbon cycling plays a major role in determining global atmospheric CO₂. A better understanding of dissolved organic matter and its constituents in the water column and how it affects marine carbon cycling is sought after. This study successfully measured osmolytes, low molecular zwitterionic compounds derived from amino acids, in...
    Resource Type:
    Masters Thesis
    Full Text:
    : ______________________________________________________ Stephen J. Giovannoni Oceanic carbon cycling plays a major role in determining

  • Single-Cell Genomics of Uncultivated Marine Bacteria

    Creator:
    Landry, Zachary
    Abstract:
    Historically, the difficulty of obtaining pure cultures of abundant marine microbial plankton has an obstacle to reconstructing the underlying mechanisms of biogeochemistry in the ocean. While a number of dominant marine species from the ocean surface have been cultured, the dominant microbial plankton of the dark ocean proved far more...
    Resource Type:
    Dissertation
    Full Text:
    : ______________________________________________________ Stephen Giovannoni Historically, the difficulty of obtaining pure cultures of abundant marine

  • Disturbances in a marine bacterioplankton system drive community assembly towards niche-based processes

    Creator:
    Vergin, Kevin L.
    Abstract:
    Over 100 monthly bacterioplankton DNA samples, from each of the surface and 200 m depths at the Bermuda Atlantic Time-series Study (BATS) site, were analyzed for community assembly processes. Correlation networks, filtered for potential autocorrelation artifacts, were constructed for each depth. Network characteristics for the two depths were remarkably similar...
    Resource Type:
    Masters Thesis
    Full Text:
    . Abstract approved: ______________________________________________________ Stephen J. Giovannoni

  • Genome-enabled investigation of the minimal growth requirements andphosphate metabolism for Pelagibacter marine bacteria

    Creator:
    Carini, Paul J.
    Abstract:
    Members of the SAR11 clade of heterotrophic α-proteobacteria are ubiquitous and abundant in the world's oceans where they are thought to play a pivotal role in the global carbon cycle. The first SAR11 bacterium cultivated in vitro, 'Candidatus Pelagibacter ubique' HTCC1062 (Ca. P. ubique), was isolated by dilution into sterile...
    Resource Type:
    Dissertation
    Full Text:
    Phosphate Metabolism for Pelagibacter Marine Bacteria Abstract approved: Stephen J. Giovannoni

  • Characterizing Growth Promoters and Inhibitors of SAR11 Pelagibacter sp. HTCC7211

    Creator:
    Lee, Connie
    Abstract:
    SAR11 is a clade of marine bacteria that are the most abundant heterotrophs in ocean surface water. They play a significant role in marine carbon cycling. Although many culturing experiments have been done on SAR11, there was no research that focused on verifying the observations about inhibition of SAR11 growth...
    Resource Type:
    Honors College Thesis
    Full Text:
    Stephen Giovannoni SAR11 is a clade of marine bacteria that are the most abundant heterotrophs

  • Culturing uncultured environmental microorganisms

    Creator:
    Connon, Stephanie A.
    Abstract:
    Research on natural environments, over the last decade, is replete with microbial diversity studies that used culture-independent approaches. The cloning and sequencing of the 16S rRNA genes has been the driving force in the expansion of awareness about the great diversity of previously undiscovered microorganisms. Well-known uncultured groups of microorganisms...
    Resource Type:
    Dissertation
    Full Text:
    Redacted for privacy ACKNOWLEDGMENTS I thank Stephen J. Giovannoni and Lewis Semprini for their

  • Microbial diversity, metabolic potential, and transcriptional activity along the inner continental shelf of the Northeast Pacific Ocean

    Creator:
    Bertagnolli, Anthony D.
    Abstract:
    Continental shelves located along eastern boundary currents occupy relatively small volumes of the world’s oceans, yet are responsible for a large proportion of global primary production. The Oregon coast is among these ecosystems. Recent analyses of dissolved oxygen at shallow depths in the water column has suggested increasing episodes of...
    Resource Type:
    Dissertation
    Full Text:
    : _____________________________________________________ Stephen J. Giovannoni Continental shelves located along eastern boundary currents occupy

  • Marine bacterioplankton abundances and distributions

    Creator:
    Morris, Robert M.
    Abstract:
    Microorganisms play key roles in ocean biogeochemistry. However, several predominant groups of uncultured bacterioplankton thought to contribute to important biogeochemical processes in the oceans are known primarily from gene cloning studies. Although these studies have greatly expanded our view of microbial diversity in the oceans, they are not quantitative and...
    Resource Type:
    Dissertation
    Full Text:
    ', Kevin L. Vergin', William A. Siebold1, Craig A. Carlson2, and Stephen J. Giovannoni' 'Department of

  • Microbial Abundance and Community Analysis in Devil’s Hole, Harrington Sound, Bermuda

    Creator:
    Denman, Carmen C.
    Abstract:
    Microbial activity within elevated CO2 and oxygen depleted environments changes with density driven stratification overturn in the seasonally anoxic region of Devil’s Hole, Bermuda. The temperature gradient developed during the summer months creates a natural laboratory to study bacterial and virus population density and microbial community dynamics in the anoxic...
    Resource Type:
    Honors College Thesis
    Full Text:
    ., Kevin L. Vergin, Jang-Cheon Cho, Michael S. Rappe, Craig A. Carlson, and Stephen J. Giovannoni

  • A genomic and proteomic characterization of the first cultured oligotrophic marine Gammaproteobacterium from the SAR92 clade

    Creator:
    Mellbye, Brett L.
    Abstract:
    High-throughput culturing (HTC) consisting of extinction culturing in autoclaved seawater has led to the isolation and characterization of many novel strains of oligotrophic marine bacteria. Strain HTCC 2207 was isolated from the Oregon coast by the HTC method. Phylogenetic analysis based on 16S rRNA gene sequence showed that this strain...
    Resource Type:
    Honors College Thesis
    Full Text:
    : _______________________________________________ Stephen J. Giovannoni High-throughput culturing (HTC) consisting of