Persistent infections with infectious pancreatic necrosis virus
(IPNV) were established in two salmonid cell lines and in live brook
trout. Virus inocula containing high concentrations of defective
interfering IPNV enhanced but were not a requirement for the establishment
of persistent infections in cell lines derived from steelhead
trout (STE-137) and...
The virulence of selected isolants of infectious hematopoietic
necrosis virus (IHNV) were differentiated and correlated with
electrophoretic mobilities of virion structural proteins by sodium
dodecylsulfate polyacrylamide gel electrophoresis. A type 1
electropherotype of IHNV was more virulent for kokanee salmon
(Oncorhynchus nerka) and a type 2 strain more virulent for...
Monoclonal antibodies (Mabs) were produced against the
myxosporean parasite, Ceratomyxa shasta, and were characterized
by indirect immunofluorescent techniques, western blot analysis,
and immunoelectron microscopy. The resulting Mabs reacted
specifically with the trophozoite and sporoblast stages of the
parasite, but not with the mature spore, indicating that certain
antigens are stage-specific....
A plan for the reestablishment of anadromous salmonid stocks into waters above the Pelton Round Butte Hydroelectric Project (PRB) located on the Deschutes River, Oregon has been under development since 1996. The PRB complex, starting at river kilometer (Rkm) 103, blocks the further upstream migration of anadromous salmonids into upper...
Myxozoans are an enigmatic group of obligately parasitic, microscopic cnidarians. They diverged from their free-living relatives over 600 million years ago and have highly reduced genomes. However, they have retained nematocyst stinging cells which characterize the phylum Cnidaria. Free-living cnidarians utilize this cellular weaponry for defense and predation whereas the...
Methylthioadenosine (MTA) is a naturally occuring nucleoside formed during
the synthesis of polyamines in mammalian cells. MTA is rapidly catabolised
by MTA phosphorylase (MTAase), eventually yielding methionine and
adenine. MTAase has been shown to be active in all normal cells, but
deficient in some cells and tissues of malignant origin....
The Baculoviridae comprise a diverse group of occluded DNA viruses that contain large double-stranded DNA genomes of 80 - 180 kb and may encode up to 180 gene products. To understand how baculoviruses replicate and process their genomes and the gene products that are involved in these events, a series...
Nearly 3000 juvenile Chinook (Oncorhynchus tshawytscha) and coho (O. kisutch) salmon captured in nearshore waters off the coasts of Washington and Oregon from 1999-2004 were tested for infection by Renibacterium salmoninarum, Nanophyetus salmincola, and skin metacercariae. First, three quantitative PCR primer/probe sets were compared for detection of R. salmoninarum; amplification...