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Purification and characterization of a DNA polymerase gamma from human trophoblast tissue

Creator:: Wong, Scott W.
Abstract:: DNA polymerase gamma has been purified over 67,500-fold from human trophoblast tissue. Purification was achieved through successive chromatographies on phosphocellulose, DEAE-cellulose, DNA-cellulose, and high pressure gel exclusion through Fractogel TSK HW55. This preparation yielded a specific activity of 81 units/mg of protein using the preferred template-primer, poly (A)-oligo (dT)₁₂₋₁₃. Analysis...
Resource Type:: Masters Thesis
Full Text:: replication and repair of nuclear DNA. DNA polymerase )( is seen predominantly in the mitochondria, where it

RNA-directed DNA polymerase activity in human placentas

Creator:: Nelson, Jay A.
Resource Type:: Dissertation
Full Text:: (Weissbach, 1975). DNA polymerase a is considered the DNA repair enzyme (Bertazzoni et al., 1976). The

The bacteriophage P1 bof protein : a corepressor whose action can both negatively and positively affect phage gene expression

Creator:: Schaefer, Timothy S.
Resource Type:: Dissertation
Full Text:: many widely separated promoter- operators. Unlike some other prokaryotic regulatory DNA binding

Escherichia coli uracil-DNA glycosylase : DNA binding, catalysis, and mechanism of action

Creator:: Shroyer, Mary Jane N.
Resource Type:: Masters Thesis
Full Text:: Excision Repair 17 1.3.1.1 Removal of Uracil from DNA 17 1.3.1.2 AP-Site Incision and Removal of 5

Effects of physiological state upon nitrosoguanidine-mediated allelic recombination in Saccharomyces cerevisiae

Creator:: Buettner, Michael John
Abstract:: Saccharomyces cerevisiae strain XS-380, a. heteroallelic diploid strain, carries six genetically analyzed loci on the left arm of chromosome VII. Nitrosoguanidine (NMG) at low concentrations was observed to cause allelic recombination (either mitotic recombination or gene conversion) of the heteroallelic loci with low lethality. The NMG-inducible heteroallelic system was used...
Resource Type:: Dissertation
Full Text:: - tion frequency. It was suggested that the cyclic activity of DNA repair enzymes may be responsible for

Control of macromolecular synthesis in Saccharomyces cerevisiae

Creator:: Wehr, Carl Timothy
Abstract:: Synthesis of total RNA, DNA, and protein was followed in a haploid yeast auxotroph during starvation for required amino acids and uracil, and during shift-up and shift-down conditions. During amino acid starvation, synthesis of macromolecular constituents was not immediately affected, reflecting the presence of large intracellular amino acid pools. Under...
Resource Type:: Dissertation
Full Text:: : Dr. Leo W. Parks Synthesis of total RNA, DNA, and protein was followed in a haploid yeast auxotroph

Characterization of Baculovirus genes involved in genome replication and processing

Creator:: Vanarsdall, Adam L.
Abstract:: The Baculoviridae comprise a diverse group of occluded DNA viruses that contain large double-stranded DNA genomes of 80 - 180 kb and may encode up to 180 gene products. To understand how baculoviruses replicate and process their genomes and the gene products that are involved in these events, a series...
Resource Type:: Dissertation
Full Text:: Construction of an AcMNPV bacmid lacking the DNA polymerase gene and construction of a repair virus

Transcriptome profiling of Nitrosomonas europaea grown singly and in co-culture with Nitrobacter winogradskyi

Creator:: Pérez, José
Abstract:: In most environments, ammonia (NH₃) is oxidized to nitrate (NO₃⁻) via the intermediate nitrite (NO₂⁻). This is a microbe-driven process involving phylogenetically distinct types of microorganisms, namely, ammonia-oxidizing bacteria (AOB) and Thaumarchaea (AOA), and nitrite-oxidizing bacteria (NOB). Nitrosomonas europaea and Nitrobacter winogradskyi are the best studied AOB and NOB, respectively,...
Resource Type:: Masters Thesis
Full Text:: genes implicated in stress responses (glutathione synthesis, DNA repair and oxidative stress e.g

Arsenic and Zinc Deficiency Induced Changes in Pancreatic Beta Cells

Creator:: Cao, Annie L.
Abstract:: Pancreatic beta cells produce and release insulin, a vital hormone that regulates blood glucose levels, and their dysfunction contributes to the development of diabetes mellitus. Dietary zinc deficiency and exposure to the toxicant inorganic arsenic have both independently been associated with diabetes, although the effects of their combination on pancreatic...
Resource Type:: Honors College Thesis
Full Text:: component of antioxidant defense proteins and DNA repair enzymes [23]. Zinc also plays an important role

Characterization of antibiotic resistant enteric bacteria that contaminate vegetables

Creator:: Salama, Maysoon
Abstract:: Previous studies revealed the high incidence of drug resistant bacteria from foods. In this study, market vegetables were tested for their contamination with antibiotic resistant (ABR) enteric bacteria. A total of 402 bacterial strains from surface vegetables (e.g., lettuce) and 321 from root vegetables (e.g., carrots) were examined for their...
Resource Type:: Masters Thesis
Full Text:: vegetables were examined for the presence of DNA plasmids. Plasmid DNA from each of these bacterial isolates

ScholarsArchive@OSU

Purification and characterization of a DNA polymerase gamma from human trophoblast tissue

RNA-directed DNA polymerase activity in human placentas

The bacteriophage P1 bof protein : a corepressor whose action can both negatively and positively affect phage gene expression

Escherichia coli uracil-DNA glycosylase : DNA binding, catalysis, and mechanism of action

Effects of physiological state upon nitrosoguanidine-mediated allelic recombination in Saccharomyces cerevisiae

Control of macromolecular synthesis in Saccharomyces cerevisiae

Characterization of Baculovirus genes involved in genome replication and processing

Transcriptome profiling of Nitrosomonas europaea grown singly and in co-culture with Nitrobacter winogradskyi

Arsenic and Zinc Deficiency Induced Changes in Pancreatic Beta Cells

Characterization of antibiotic resistant enteric bacteria that contaminate vegetables

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