Index Catalog // ScholarsArchive@OSU

The distribution and influence of sterols in yeast membranes

Creator:: Bottema, Cynthia D. K.
Abstract:: Plasma membranes from Saccharomyces cerevisiae were prepared by a new procedure involving lyticase treatment of the yeast cells. The plasma membranes were right-side out, closed vesicles of uniform appearance with a sterol to phospholipid molar ratio of 0.365. The thermotropic behavior of these plasma membranes from wild-type yeast and from...
Resource Type:: Dissertation
Full Text:: membranes AN ABSTRACT OF THE THESIS OF Cynthia D. K. Bottema for the degree of Doctor of Philosophy in

Performance of Oregon derived malolactic bacteria : studies on storage stability and their use in the wine industry

Creator:: Micheals, Nancy J.
Abstract:: Commercial vats of Chardonnay and Pinot Noir were inoculated during yeast fermentation with starter cultures containing 10⁷ - 10⁹ cfu/ml of the malolactic bacteria Leuconostoc oenos Erla and Ey2d in lots ranging from 55 to 4,000 gallons. Chardonnay inoculated with Ey2d completed MLF in an average of 56 days at...
Resource Type:: Masters Thesis
Full Text:: wine samples in the lab trials rose consistently with the highest values associated with wine lots

Selection of phage insensitive lactic streptococcal strains for use in cheddar cheese manufacture

Creator:: Daniell, Suzanne D.
Abstract:: A survey was conducted of six North American cheese plants to determine levels of bacteriophages present. Fifty-nine lactic streptococcal host bacterial strains were utilized in the survey. Phage levels were generally lower in plants employing modern manufacturing techniques than in plants employing traditional manufacturing techniques; this difference was attributed to...
Resource Type:: Masters Thesis
Full Text:: plants that participated in the study. The technical assistance of Cynthia Morrow and Diane Zielinsky is

The development of Phytophthora cactorum as a model system for the study of sterol metabolism

Creator:: Gonzales, Robert A.
Abstract:: Phytophthora cactorum lacks sterol synthesis capability. Nevertheless, it exhibits vegetative growth in the total absence of sterol. Supplementation of the growth medium with sterol, however, resulted in a three-fold increase in cell yield. This increase was shown by whole cell and cell free experiments to be the consequence of altered...
Resource Type:: Dissertation
Full Text:: and encouragement. Special gratitude is due Cynthia Bottema for her assistance and many stimulating

Coordinated regulation of plasma membrane lipids in Saccharomyces cerevisiae

Creator:: Low, Christopher S. F.
Abstract:: Plasma membranes isolated from a yeast sterol auxotroph (RD5-R) grown on 1,5, and 15 pg m1⁻¹ exogenous concentrations of sterol showed no discontinuity in plots of steady-state fluorescence anisotropy. Liposomes constructed from phospholipid and sterol extracted from RD5-R grown on different sterols indicated exogenously supplied sterol modulated cellular phospholipids such...
Resource Type:: Dissertation
Full Text:: constructive comments and devil's advocate criticisms from Drs. Tom Lewis, Cynthia Bottema, and Russell

Characterization of an immunoreactive region of the major capsid protein for infectious pancreatic necrosis virus

Creator:: Barrie, Rosemary Jean
Abstract:: DNA fragments of the gene for VP2, the major capsid protein of Infectious Pancreatic Necrosis Virus (IPNV), were expressed in Escherichia coli and characterized immunologically with antisera made to different serotypes of IPNV. The entire VP2 gene was excised from the plasmid pUC19/A+SΔK and fragmented with restriction enzyme Sau3A1. The...
Resource Type:: Masters Thesis
Full Text:: . Computational analysis to derive hydropathicity plots of the protein sequence (Rose, 1978) and to make surface

Characterizing Mycobacterium avium subspecies hominissuis (MAH) Microaggregate Induction of Innate Immunity

Creator:: Keefe, Bailey F.
Abstract:: Bacterial aggregation is a strategy employed by many pathogens to establish infection. Mycobacterium avium subsp. hominissuis (MAH) undergoes a phenotypic change, microaggregation, when exposed to the respiratory epithelium. This aggregation is an important step in the pathogenesis of the infection, laying the foundation for biofilm formation. We therefore compared how...
Resource Type:: Masters Thesis
Full Text:: has pushed me to the finish line. I also want to give Dr. Sasha Rose special thanks for training me

The role of chlamydial inclusion membrane proteins in host-pathogen interaction and the development of novel methods for studying chlamydial biology

Creator:: Alzhanov, Damir T.
Abstract:: The majority of our modern understanding of bacterial pathogenesis is based on the strategy that involves screening bacterial genomes for the presence of the genes encoding pathogenic factors, and analysis of these genes via forward and reverse genetics. Chlamydiae represent a unique group of pathogenic bacteria in which it is...
Resource Type:: Dissertation
Full Text:: Dr. Dennis E. Hruby for their time, advice, and support. I also want to thank Dr. Cynthia

Consequences of Influenza A Virus on Pneumococcal Biofilm Formation and Stability in the Absence of Host Factors

Creator:: Nutter, Laura
Abstract:: Streptococcus pneumoniae and Influenza A Virus both primarily infect the upper respiratory tract, with more severe disease occurring during coinfection than would be caused by either pathogen independently. Until recently, characterization of the interactions between these two pathogens has occurred exclusively in the context of a host or using host-derived...
Resource Type:: Masters Thesis
Full Text:: overlaying the host epithelial cells (Feldman et al, 1992). During commensal infections, the bacteria do not

Characterization of the Sodium Transporting NADH:Quinone Oxidoreductase (NQR) in Vibrio cholerae

Creator:: Fassio, Sara R.
Abstract:: Vibrio cholerae is the causative agent of cholera, a serious diarrheal disease in developing countries. V. cholerae has a unique redox driven respiration-linked sodium pump, Na⁺ translocating NADH:quinone oxidoreductase (NQR). Several reports previously showed that NQR plays an important role in virulence, metabolism, and sodium homeostasis of V. cholerae. This...
Resource Type:: Honors College Thesis
Full Text:: at the early log growth phase (0-4 hours) (Fig. 2C). However, at the late-log growth phase (8 hours

ScholarsArchive@OSU

The distribution and influence of sterols in yeast membranes

Performance of Oregon derived malolactic bacteria : studies on storage stability and their use in the wine industry

Selection of phage insensitive lactic streptococcal strains for use in cheddar cheese manufacture

The development of Phytophthora cactorum as a model system for the study of sterol metabolism

Coordinated regulation of plasma membrane lipids in Saccharomyces cerevisiae

Characterization of an immunoreactive region of the major capsid protein for infectious pancreatic necrosis virus

Characterizing Mycobacterium avium subspecies hominissuis (MAH) Microaggregate Induction of Innate Immunity

The role of chlamydial inclusion membrane proteins in host-pathogen interaction and the development of novel methods for studying chlamydial biology

Consequences of Influenza A Virus on Pneumococcal Biofilm Formation and Stability in the Absence of Host Factors

Characterization of the Sodium Transporting NADH:Quinone Oxidoreductase (NQR) in Vibrio cholerae

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