Diseases caused by parasites are recognized as significant sources of mortality in wild fish populations. I assessed the impacts of multispecies parasitism on a threatened stock of juvenile coho salmon (Oncorhynchus kisutch). A crucial prerequisite to this research was proper identification of parasites, which can be difficult for species lacking...
During the development of the media for the identification and
differentiation of Clostridium perfringens and C. botulinum types A,
B and E, 16 different double layer media were prepared and tested
with known cultures of 28 C. botulinum strains, 20 C. perfringens
strains and 11 other clostridial species.
The semisolid...
The chemolithoautotrophic nitrite oxidizing bacteria (NOB) participate in the
biogeochemical cycling of nitrogen by catalyzing and conserving energy from the
oxidation of nitrite (NO₂-) to nitrate (NO₃-) via a nitrite oxidoreductase (NXR). The
main objective of this work was to comparatively annotate and analyze the genome
sequences of Nitrobacter winogradskyi...
Proper immune function is indispensable, as failure to mount an immune response against a pathogen can lead to serious complications or even death. T cells act by enhancing the activation of phagocytic cells as well as the activation of B cells. Their widespread influence on an immune response makes optimal...
Lactococcus lactis subsp. cremoris Ropy 352 and L. lactis
subsp. cremoris Hollandicus produce an exopolysaccharide (EPS) that
imparts commercially desirable textural and rheological properties
to fermented milk products. This ropy phenotype is expressed under
specific environmental conditions. A mucoid EPS phenotype, also
expressed under specific environmental conditions, but not involved...
Wild fish populations are typically infected with a variety of micro- and macroparasites that may affect fitness and survival, however, there is little published information on parasite distribution in wild juvenile salmonids in three upper tributaries of the Willamette River, OR. The objectives of this survey were to document (1)...
Nearly 3000 juvenile Chinook (Oncorhynchus tshawytscha) and coho (O. kisutch) salmon captured in nearshore waters off the coasts of Washington and Oregon from 1999-2004 were tested for infection by Renibacterium salmoninarum, Nanophyetus salmincola, and skin metacercariae. First, three quantitative PCR primer/probe sets were compared for detection of R. salmoninarum; amplification...
Little is known about the virulence factors of Renibacterium
salmoninarum, the causative agent of salmonid bacterial kidney disease.
The predominant protein produced by R. salmoninarum in broth culture or
during infection is a 57/58 kDa protein (p57) which is associated with strain
virulence. In this study monoclonal antibodies (MAbs) to...
Limnology is undergoing a transition to high-throughput -omic analysis of freshwater
bacterial communities. An important first step in making the transition is to
characterize several genomes that can be used as references to guide metagenome
assembly and analysis. Here I characterize four new freshwater cyanobacterial
genomes, a pair of lake...