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Filtering by: Creator Getzin, Louis W. Remove constraint Creator: Getzin, Louis W. Degree Field Microbiology Remove constraint Degree Field: Microbiology

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  • Significance of pigments in radiation resistance of Micrococcus radiodurans

    Creator:
    Krabbenhoft, Kenneth Louis
    Abstract:
    An ecological study of a microorganism similar to that isolated in 1956 and designated as Micrococcus radiodurans indicated that the organism could be isolated from several different environments. By taking advantage of its radio-resistivity, high levels of gamma radiation were used in the isolation procedures to inactivate most of the...
    Resource Type:
    Dissertation
    Full Text:
    Micrococcus radiodurans AN ABSTRACT OF THE THESIS OF Kenneth Louis Krabbenhoft for the Ph. D, in

  • Biosynthesis of yeast sterols

    Creator:
    Turner, Jan Ross
    Abstract:
    An enzyme system from cell-free yeast homogenates mediating the transfer of the methyl group from S-adenosylmethionine-methyl-C¹⁴ or methionine-methyl-C¹⁴ to C-28 of ergosterol has been studied. The effects of divalent cations, suithydryl inhibitors and reducing substances on the activity of the enzyme system have been investigated. It was established that for...
    Resource Type:
    Masters Thesis
    Full Text:
    Dr. L. W. Parks first for his contagious enthusiasm and first also for his guidance in this study

  • Studies on the fatty acid and lipid composition of Clostridium botulinum

    Creator:
    McCollough, Marian Lorraine
    Abstract:
    Routine studies of bacterial fatty acid and lipid composition, made possible by recently developed techniques, have brought about an increasing interest in the physiological significance of these compounds. The purpose of this study was to characterize the fatty acids of the vegetative cells of Clostridium botulinum 33A, as the first...
    Resource Type:
    Masters Thesis
    Full Text:
    . Arthur W. Anderson, for giving me the opportunity to conduct this study and for his guidance and

  • The role of S-adenosylmethionine in the biosynthesis of ergosterol and related sterols in Saccharomyces cerevisiae

    Creator:
    Turner, Jan Ross
    Abstract:
    An enzyme system was prepared from Saccharomyces cerevisiae, strain MCC, which carries out the transfer of the methyl group from S-adenosylmethionine or methionine to the side chain of yeast sterols. The cell-free system has been shown to be incapable of synthesizing ergosterol but carries out the synthesis of two as...
    Resource Type:
    Dissertation
    Full Text:
    by Ruth Baines ACKNOWLEDGMENTS I want to express my appreciation to Dr. L. W. Parks for his

  • Carbohydrate metabolism in lactic streptococci with special reference to galactose

    Creator:
    Lee, Donald Royce
    Abstract:
    Phosphorylation of free galactose by lactic streptococci was mediated by an adenosine triphosphate (ATP) dependent kinase which was repressed by glucose. The phosphoenolypyruvate (PEP) phosphotransferase system (PTS) was not involved in transport of galactose, The conversion of free galactose to glucose was also demonstrated. A key Leloir pathway enzyme, uridine...
    Resource Type:
    Dissertation
    Full Text:
    LACTIC STREPTO- COCCI WITH SPECIAL REFERENCE TO GALACTOSE Redacted for Privacy Abstract approved: Dr. W

  • Aspects of ergosterol biosynthesis in Saccharomyces cerevisiae

    Creator:
    Monner, David Anthony
    Abstract:
    The regulation of ergosterol biosynthesis in yeast has been studied. The system has been examined for the presence of an end-product inhibition. Both anaerobically and aerobically grown cells have been examined for this effect. A feedback inhibition of sterol synthesis has been shown, but it appears to be a secondary...
    Resource Type:
    Dissertation
    Full Text:
    ERGOSTEROL BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE Abstract approved: Leo W. Parks The regulation of

  • Recovery of Saccharomyces cerevisiae from ethanol-induced growth inhibition

    Creator:
    Walker, Helen M. (Helen Marguerite), 1961-
    Abstract:
    Ethanol caused altered mobility of the lipophylic probe, 1,6-diphenyl-1,3,5-hexatriene in plasma membrane preparations of Saccharomyces cerevisiae. Because lipids had been shown to protect yeast against ethanol toxicity, sterols, fatty acids, proteins, and combinations of these were tested, but protection from growth inhibition was not seen. Ethanol-induced prolonged lag periods and...
    Resource Type:
    Masters Thesis
    Full Text:
    cerevisiae from Ethanol-Induced Growth Inhibition Abstract approved: Redacted for Privacy Leo W. Parks

  • Identification of volatiles from a swine center using porous polymers and cold traps

    Creator:
    Kelly, Michael David
    Abstract:
    This project was initiated to find a practical way of identifying the volatiles and monitoring their relative concentrations. Special attention was given to the identification of the organic acids. Volatile compounds produced in a swine confinement building were trapped by porous polymers, Porapak Ω and Tenax GC, and identified by...
    Resource Type:
    Masters Thesis
    Full Text:
    (Sigma Chemical Co., St. Louis, Mo.) coated on 100/120 mesh Chromosorb W (F & M Scientific, Avondale

  • Sterol 24(28) methylene reductase in Saccharomyces cerevisiae

    Creator:
    Neal, William Daniel
    Abstract:
    A cell free extract from Saccharomyces cerevisiae containing the enzyme that causes reduction of the 24(28)methylene group on the side chain of ergosterol has been studied. Optimal conditions for the 24(28)methylene reductase were obtained. The enzyme activity was increased in cells grown with ethanol as the substrate. The Km was...
    Resource Type:
    Masters Thesis
    Full Text:
    (28)Methylene Reductase in Saccharomyces cerevisiae Abstract approved: Redacted for Privacy Leo W

  • Morphologic, molecular and antigenic characteristics of Bacteroides nodosus

    Creator:
    Gradin, Joseph Lloyd
    Resource Type:
    Dissertation
    Full Text:
    Bactt.!idel'' sus Pili Abstract approved: Redacted for privacy Alvin W. Smith V Bacteroides nodosus is