Oceanic carbon cycling plays a major role in determining global atmospheric CO₂. A better understanding of dissolved organic matter and its constituents in the water column and how it affects marine carbon cycling is sought after. This study successfully measured osmolytes, low molecular zwitterionic compounds derived from amino acids, in...
Historically, the difficulty of obtaining pure cultures of abundant marine
microbial plankton has an obstacle to reconstructing the underlying
mechanisms of biogeochemistry in the ocean. While a number of dominant
marine species from the ocean surface have been cultured, the dominant
microbial plankton of the dark ocean proved far more...
Over 100 monthly bacterioplankton DNA samples, from each of the surface and 200 m depths at the Bermuda Atlantic Time-series Study (BATS) site, were analyzed for community assembly processes. Correlation networks, filtered for potential autocorrelation artifacts, were constructed for each depth. Network characteristics for the two depths were remarkably similar...
Members of the SAR11 clade of heterotrophic α-proteobacteria are ubiquitous and abundant in the world's oceans where they are thought to play a pivotal role in the global carbon cycle. The first SAR11 bacterium cultivated in vitro, 'Candidatus Pelagibacter ubique' HTCC1062 (Ca. P. ubique), was isolated by dilution into sterile...
Candidatus Pelagibacter ubique is the first cultured representative of the SAR11 clade, a clade that is found throughout the oceans and accounts for approximately 25% of all bacterial cells [1]. It has a streamlined genome that is the smallest of any known free-living organism. In this study the complete genome...
High-throughput culturing (HTC) consisting of extinction culturing in autoclaved seawater has led to the isolation and characterization of many novel strains of oligotrophic marine bacteria. Strain HTCC 2207 was isolated from the Oregon coast by the HTC method. Phylogenetic analysis based on 16S rRNA gene sequence showed that this strain...
Microorganisms play key roles in ocean biogeochemistry. However, several
predominant groups of uncultured bacterioplankton thought to contribute to
important biogeochemical processes in the oceans are known primarily from gene
cloning studies. Although these studies have greatly expanded our view of
microbial diversity in the oceans, they are not quantitative and...
A new high-throughput culturing (HTC) method using a low nutrient
heterotrophic medium (LNHM) has led to the isolation of many novel strains of
oligotrophic bacteria from marine ecosystems. Four strains belonging to a single
dade, HTCC2151, HTCC218OT, HTCC2178T and HTCC2188T, were isolated
from the coast of Oregon by the HTC...
Symbiotic relationships of bacteria with higher organisms are
commonly observed in nature; however, the functional role of these
relationships is only rarely understood. This is particularly evident in
epibiotic bacterial associations in the marine environment where the bacteria
are often a diverse ensemble of microorganisms, thus complicating the
identification of...