High-throughput culturing (HTC) consisting of extinction culturing in autoclaved seawater has led to the isolation and characterization of many novel strains of oligotrophic marine bacteria. Strain HTCC 2207 was isolated from the Oregon coast by the HTC method. Phylogenetic analysis based on 16S rRNA gene sequence showed that this strain...
Candidatus Pelagibacter ubique is the first cultured representative of the SAR11 clade, a clade that is found throughout the oceans and accounts for approximately 25% of all bacterial cells [1]. It has a streamlined genome that is the smallest of any known free-living organism. In this study the complete genome...
Microorganisms play key roles in ocean biogeochemistry. However, several
predominant groups of uncultured bacterioplankton thought to contribute to
important biogeochemical processes in the oceans are known primarily from gene
cloning studies. Although these studies have greatly expanded our view of
microbial diversity in the oceans, they are not quantitative and...
A new high-throughput culturing (HTC) method using a low nutrient
heterotrophic medium (LNHM) has led to the isolation of many novel strains of
oligotrophic bacteria from marine ecosystems. Four strains belonging to a single
dade, HTCC2151, HTCC218OT, HTCC2178T and HTCC2188T, were isolated
from the coast of Oregon by the HTC...
Symbiotic relationships of bacteria with higher organisms are
commonly observed in nature; however, the functional role of these
relationships is only rarely understood. This is particularly evident in
epibiotic bacterial associations in the marine environment where the bacteria
are often a diverse ensemble of microorganisms, thus complicating the
identification of...
The objective of this project was to use ribosomal RNA genes, cloned
from natural populations of Sargasso Sea bacterioplankton, as markers for
picoplankton diversity. It is widely recognised that a majority of
microorganisms have yet to be cultivated, and therefore much of extant
microbial diversity remains unknown (50). The method...
Members of the SAR11 clade of heterotrophic α-proteobacteria are ubiquitous and abundant in the world's oceans where they are thought to play a pivotal role in the global carbon cycle. The first SAR11 bacterium cultivated in vitro, 'Candidatus Pelagibacter ubique' HTCC1062 (Ca. P. ubique), was isolated by dilution into sterile...
The starvation-survival patterns of 16 freshly isolated open
ocean bacteria and in a psychrophilic Vibrio sp., Ant-300, were
determined. These isolates displayed three survival patterns as
determined by viable cell count. These were (a) a rapid increase
followed by a decrease to a constant value (pattern of Ant-300 and
7...
The physiological responses of populations of a marine
Pseudomonas sp. to nutrient starvation were observed for a period
of 40 days. During the first 25 days of starvation the viability
of the population decreased by 99.9 percent, but thereafter the
cultures maintained approximately 5 x 10⁵ viable cells per ml...