Salmonid B lymphocytes were stimulated both in vivo and in vitro
and shown to produce antibody specific for the immunogen. A passive
hemolytic plaque assay was employed to demonstrate B cells producing
specific antibody. Characterization of plaque forming cells (PFCs)
involved sequential inhibition with a series of graded concentrations
of...
The in vitro effects of cortisol in regulating salmonid B cell functions were
investigated. B cells at three distinct stages of differentiation were examined for
cortisol sensitivity. B lymphocyte responses were examined 1) during the early stage
of the precursor, 2) during the intermediate stage of differentiation associated with
clonal...
During the development of the media for the identification and
differentiation of Clostridium perfringens and C. botulinum types A,
B and E, 16 different double layer media were prepared and tested
with known cultures of 28 C. botulinum strains, 20 C. perfringens
strains and 11 other clostridial species.
The semisolid...
Humans and viral disease are inextricably intertwined. Viral disease plays an immeasurable role in human life, from the disease and economic burden associated with every facet of contending with human viral disease, to managing the consequences of viral disease in organisms important to our food supply, economy, and entertainment. The...
The vitamin B₁₂ concentrations in both the water and sediments
of Klamath Lake were found to vary significantly during a
one-year period. The patterns of B₁₂ distribution were remarkably
similar among the three main sites. In all cases there was an extremely
sharp gradation in concentration in relation to depth...
Bdellovibrio bacteriovorus 100 and B. stolpii UKi-2 each produce
two extracellular proteases which attack Azocoll (collagen)
while B. starrii A3.12 produces at least four such proteolytic
enzymes. Under standard growth and assay conditions each Bdellovibrio
species produces a unique amount of proteolytic activity. The
decreasing order of enzyme units was...
A large number of bacterial and yeast isolates were obtained
from meat and poultry processing plants by swab and contact plate
sampling methods. Most of the isolates were subjected to a brief
taxonomic study and listed according to their sources. Representative
food spoilage, indicator and pathogenic organisms were identified
to...
Three closely related isolates belonging to the A₁ serotype of infectious pancreatic
necrosis virus (IPNV) were selected for comparison, to provide insight into the nature of
variation in the virulence of IPN viruses. Brook trout fry (Salvelinus fontinalis) were
experimentally infected with the three isolates by immersion. Cumulative mortalities over...