The in vitro effects of cortisol in regulating salmonid B cell functions were
investigated. B cells at three distinct stages of differentiation were examined for
cortisol sensitivity. B lymphocyte responses were examined 1) during the early stage
of the precursor, 2) during the intermediate stage of differentiation associated with
clonal...
Current detection methods for bacterial contamination rely on structure based detection of proteins and nucleic acids. While these methods are easy to use and reliable, they cannot evaluate the toxicity of a sample and the potential to cause disease. Previously, erythrophore cells derived from Betta splendens had been suggested as...
Historically, the difficulty of obtaining pure cultures of abundant marine
microbial plankton has an obstacle to reconstructing the underlying
mechanisms of biogeochemistry in the ocean. While a number of dominant
marine species from the ocean surface have been cultured, the dominant
microbial plankton of the dark ocean proved far more...
Cell-based biosensors are function-based detectors that use the physiological response of a living cell to sense biologically stimulating agents. This emerging technology extends the application of current detection methods by reporting on the toxicity of a sample and the potential to cause disease. Previously, Betta splendens erythrophores have been described...
The psychrophilic marine Vibrio sp., ANT-300, was able to utilize a variety of carbon sources that can be considered typical for the marine environment. The organism grew abundantly on glucose and serine. Chitin was hydrolyzed even when it was offered as the only carbon source, while no growth occurred on...
Detection of both biological and chemical environmental toxicants is essential in the assessment of risk to human health. Cell-based biosensors are capable of activity- based detection of toxicity. Chromatophore cells, responsible for the pigmentation of poikilothermic animal, have shown immense potential as cell-based biosensors in the detection of a broad...
The ubiquitous presence of the coliform bacterium
Klebsiella in botanical environments has been demonstrated.
In this study, Klebsiella were found to multiply and
colonize growing radish plants following contamination of
seeds. All 29 cultures of Klebsiella tested from 5 different
environments were capable of growth to 10⁶-10⁷ cells/gram
of plant...
Agronomic and microbiological studies were conducted on "Anchor" alfalfa (Medicago sativa L.) established from uninoculated seed on three Aridisols in central Oregon. Significant responses to 230 Kg N per ha (NH₄NO₃), which was applied in mid-April and after each first and second harvests, were observed in yield parameters of the...
Bacterial contamination of food poses a great risk to human health worldwide. A
chromatophore cell-based biosensor, utilizing B. splendens erythrophore cells, is an
emerging technology that has shown potential to detect bacterial toxicity based on
function-dependent mechanisms. Previous studies have investigated the response of
erythrophore cells to foodborne pathogens, pesticides,...