Efficacious vaccines were developed for the control of Vibrio
anguillarum, the etiological agent of vibriosis in salmonid fish.
These bacterins can be administered either orally or parenterally.
It was determined that both formalin-killed lyophilized whole cells
and wet-packed whole cells of the organism are effective oral immunogens.
Intraperitoneal injection of...
Immunity in juvenile coho salmon (Oncorhynchus kisutch) was
evaluated after immunization against vibriosis by parenteral, oral or
immersion methods. Relative levels of immunity were determined by
protection of fish from experimental water-borne challenges with
Vibrio anguillarum and by serum agglutination titers. Coho salmon
eggs were exposed to bacterin during artificial...
Unfertilized ova from spring chinook salmon (Oncorhynchus
tshawytscha) were examined for the presence of classical salmonid
inmmunoglobulin. Immunodiffusion techniques in which rabbit anti-salmonid
immunoglobulin was reacted against an ova homogenate
and rabbit anti-ova homogenate was reacted against immune chinook
serum failed to detect classical-type antibodies within these eggs.
A lectin...
After parenteral immunization with Aeromonas salmonicida
cells, the agglutination activity in juvenile coho salmon (Oncorhynchus
kisutch) serum was associated with the macroglobulin serum fraction.
Antibody activity was diminished by 30 minutes exposure to 50 C
but not 45 C. Reduction with 2-mercaptoethanol and alkylation
markedly lowered but did not completely...
Experiments were designed to more precisely evaluate the effects of water temperature on the progress of bacterial kidney disease ( BKD) in three species of salmonid fishes. Infections were produced by intraperitoneal injection of suspensions of the causative organism into fish held at seven temperatures ranging from 3.9 to 20.5°C....
Twenty-two isolates of Flexibacter columnaris were
collected from different species of fish from a wide
geographic range. Shieh medium was selected and modified
to provide satisfactory growth of F. columnaris strains.
Although there were three phenotypic variations in colony
morphology among the strains observed, the environmental
and biochemical characteristics as...