During the development of the media for the identification and
differentiation of Clostridium perfringens and C. botulinum types A,
B and E, 16 different double layer media were prepared and tested
with known cultures of 28 C. botulinum strains, 20 C. perfringens
strains and 11 other clostridial species.
The semisolid...
Investigations into the phylogeny, genome size, and karyotype of microsporidian Nadeispora canceri were initiated to further characterize the organism. Isolates of N. canceri spores were obtained from both Dungeness (Cancer magisrer) and red rock crabs (Cancer productus). Analysis of the ssu rDNA sequence from spore isolates of the two crab...
The characteristics of an inhibitor of infectious pancreatic necrosis virus (IPNV)
found in normal rainbow trout serum (RTS) were studied. The serum inhibitor had a
molecular weight of approximately 150 kDa and was dependent on divalent cations,
either Ca²⁺ or Mg²⁺. It was stable at temperatures up to 50°C and...
Some enzymes of the citric acid cycle and glycolytic pathway
in cell-free extracts of Vibrio marinus MP-1 were compared for
thermal lability. After one hour of moderate temperature exposure,
enzymes of both pathways rapidly lost catalytic activity. For all
but one enzyme, 50 percent remaining activity occurred near an
averaged...
Three closely related isolates belonging to the A₁ serotype of infectious pancreatic
necrosis virus (IPNV) were selected for comparison, to provide insight into the nature of
variation in the virulence of IPN viruses. Brook trout fry (Salvelinus fontinalis) were
experimentally infected with the three isolates by immersion. Cumulative mortalities over...
A large number of bacterial and yeast isolates were obtained
from meat and poultry processing plants by swab and contact plate
sampling methods. Most of the isolates were subjected to a brief
taxonomic study and listed according to their sources. Representative
food spoilage, indicator and pathogenic organisms were identified
to...
Disseminated neoplasia (DN) is a proliferative cell disorder that occurs in the
circulatory system of bivalves. The condition is progressive and lethal. At least 15
species of bivalves over a wide range of geographic locations have been reported to
contract DN. Prevalence levels of disseminated neoplasia can reach up to...
In an attempt to determine the natural habitat of Streptococcus
lactis, Streptococcus cremoris, and Streptococcus diacetilactis 27
different species of vegetables, 18 species of fruits and many individual
cow raw milk samples were examined.
S. lactis was found to occur on potatoes, corn, cucumbers,
peas, beans, and cantaloupe. In each...