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Filtering by: Creator Wilson, T. R. C. (Thomas Randall Carson), 1884- Remove constraint Creator: Wilson, T. R. C. (Thomas Randall Carson), 1884- Degree Field Microbiology Remove constraint Degree Field: Microbiology

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  • Molecular characterization of the MX genes of rainbow trout (Oncorhynchus mykiss)

    Creator:
    Trobridge, Grant David
    Abstract:
    The Mx protein family of rainbow trout (Oncorhynchus mykiss) was characterized at the molecular level. With primers derived from a partial sequence of Perch (Perca fluviatis) Mx genomic DNA, a partial rainbow trout Mx genomic DNA sequence was obtained by polymerase chain reaction (PCR) amplification. All salmonid fish investigated contained...
    Resource Type:
    Dissertation
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    of Rainbow Trout (Oncorhynchus vkiss). Abstract approved: Jo-Ann C. Leong The Mx protein

  • The Influence of Microbial Communities on Spatiotemporal Elemental Cycling in Coastal Margins Revealed by Community- and Population-level Genomics and Proteomics

    Creator:
    Kieft, Brandon P.
    Abstract:
    Biotic and abiotic processes at continent-ocean interfaces cycle a disproportionate mass of carbon and nutrients relative to their global surface area, and microbial activity is a principal determinant of organic and inorganic matter flux in these transition zones. Most studies using modern high-throughput ‘omics techniques to link microorganisms with costal...
    Resource Type:
    Dissertation
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    experiments, performed experiments, data analysis, manuscript edits Bryon C. Crump: Methodological expert

  • Chromatophores as cell-based biosensors for the detection of chemically and biologically toxic substances

    Creator:
    Dukovcic, Stephanie R.
    Abstract:
    Cell-based biosensors are function-based detectors that use the physiological response of a living cell to sense biologically stimulating agents. This emerging technology extends the application of current detection methods by reporting on the toxicity of a sample and the potential to cause disease. Previously, Betta splendens erythrophores have been described...
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    Dissertation
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    AN ABSTRACT OF THE DISSERTATION OF Stephanie R. Dukovcic for the degree of Doctor of Philosophy

  • Harnessing Heterogeneity in Human Gut Microbiome Data

    Creator:
    Tataru, Christine
    Abstract:
    The focus of this thesis is to design, characterize, and apply novel computational methods and molecular systems to interrogate heterogeneous human gut microbiome-related phenomena. In Chapter 2, I design, implement, and characterize a method for embedding co-occurrence patterns derived from massive 16s amplicon datasets. I use this method to 1....
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    Dissertation
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    . . . . . . . . . . . . . . . . . . . . . . . . 61 3 GMEmbeddings: an R package to apply embedding techniques to micro- biome data 63 3.1 Abstract

  • Chemical and Genetic Investigations of Toxic Freshwater Cyanobacterial Blooms for Improved Monitoring and Understanding of Lake Ecology

    Creator:
    Collart, Lindsay P.
    Abstract:
    Toxin production by cyanobacterial harmful algal blooms (cyanoHABs) in freshwater systems has lasting ecological and human impacts. Nutrients, light availability, hydrology, and microbial community composition impact the frequency and intensity of toxic cyanoHABs. Climate change will exacerbate toxic cyanoHABs, making real time and predictive monitoring a vital tool for managing...
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    Dissertation
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    chemical composition (Randall et al. 2019). Sediment effects on HABs are rarely addressed. They are also

  • Ion and salinity effects on induction and function of galactose permease in an Antarctic psychrophilic marine bacterium

    Creator:
    Hayasaka, Steven Shin
    Abstract:
    A number of Antarctic marine psychrophilic bacteria were isolated having the ability to grow and accumulate ¹⁴ C-TMG only at temperatures below 20 C. It was shown that galactose rather than lactose was the primary inducer and utilizable substrate for the permease system. Further studies with a Vibrio species (Ant-12),...
    Resource Type:
    Dissertation
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    the ability to grow and accumulate 14 C-TMG only at temperatures below 20 C. It was shown that

  • Fecal Bacteroidetes host distributions and environmental source tracking

    Creator:
    Dick, Linda K.
    Abstract:
    Contamination of recreational and shellfish waters with fecal pollution is a major water quality issue with associated economic impacts and human health risks. Reliable fecal source identification and rapid, quantitative analyses are essential components of risk assessment. Enteric bacteria that are endemic to specific hosts have a potential role as...
    Resource Type:
    Dissertation
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    for Microbiology, Washington, D.C. Desmarais, T. R., H.M. Solo-Gabriele, and C. J. Palmer. 2001

  • B-cell heterogeneity in salmonids

    Creator:
    Irwin, Michael J.
    Abstract:
    Salmonid B lymphocytes were stimulated both in vivo and in vitro and shown to produce antibody specific for the immunogen. A passive hemolytic plaque assay was employed to demonstrate B cells producing specific antibody. Characterization of plaque forming cells (PFCs) involved sequential inhibition with a series of graded concentrations of...
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    Dissertation
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    pathway begins with the processing of antigen, by the macrophage, for presentation to the T cell (Thomas

  • Survival and persistence of Bacteroidales human and ruminant specific fecal markers and occurrence with fecal pathogens

    Creator:
    Walters, Sarah P.
    Abstract:
    Aquatic fecal contamination from non-point sources impairs environmental health and serves as a vehicle for transmission of waterborne disease, resulting in economic losses worldwide. Accurate methods of diagnosing fecal pollution and its source are needed to prevent human exposure, remediate pollution, and reduce economic impacts. In order to obtain this...
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    Dissertation
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    detected when pathogens are present, persist less than 14 days in natural river water at 13°C, and the

  • Carbohydrate metabolism in lactic streptococci with special reference to galactose

    Creator:
    Lee, Donald Royce
    Abstract:
    Phosphorylation of free galactose by lactic streptococci was mediated by an adenosine triphosphate (ATP) dependent kinase which was repressed by glucose. The phosphoenolypyruvate (PEP) phosphotransferase system (PTS) was not involved in transport of galactose, The conversion of free galactose to glucose was also demonstrated. A key Leloir pathway enzyme, uridine...
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    Dissertation
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    continued help this research would never have been possible. Appreciation is also expressed to: Dr. P. R