Index Catalog // ScholarsArchive@OSU

An emerging role for the exocyst : plant morphogenesis

Creator:: Cole, Rex A.
Abstract:: Plants develop a vast array of cell shapes and sizes by selectively modifying their surrounding cell walls to expand in some regions and not in others. This process of morphogenesis requires the delivery of secretory vesicles to specific locations at the cell periphery, where exocytosis adds new membrane and proteins...
Resource Type:: Dissertation
Full Text:: protein complex, the exocyst. Prior to my work, genes encoding all eight components of the exocyst had

Binding and assembly of H5 (and the globular domain of H5) onto DNA

Creator:: Carter, George John
Abstract:: In order to better characterize linker histone interactions with DNA, avian erythrocyte-specific linker H5 and the trypsin-resistant globular domain of H5 (GH5) were used in DNA binding studies. To begin, H5 displayed a considerably higher binding affinity for DNA than the isolated globular domain (GH5), supporting the importance of the...
Resource Type:: Dissertation
Full Text:: protein-DNA contact. Linker histones also induce DNA-protein aggregation in a process that was found to

Biochemical characterization and mutational analysis of human uracil-DNA glycosylase

Creator:: Chen, Cheng-Yao
Abstract:: PCR-based codon-specific random mutagenesis and site-specific mutagenesis were performed to construct a library of 18 amino acid changes at Arg276 in the conserved leucine-loop of the core catalytic domain of human uracil-DNA glycosylase (UNG). Each Arg276 mutant was then overproduced in E. coli cells and purified to apparent homogeneity by...
Resource Type:: Dissertation
Full Text:: R276 mutant proteins formed a stable complex with the uracil-DNA glycosylase inhibitor protein (Ugi

Creation of an efficient protein expression and secretion system in the gram-positive bacterial vector, Streptococcus gordonii

Creator:: Myscofski, Dawn M.
Abstract:: Streptococcus gordonii (S. gordonii) is being developed as a Gram-positive expression vector for the expression of heterologous proteins. This bacterial expression system takes advantage of the natural pathway of protein export and anchoring on the surface of the bacterium. The expression of heterologous proteins relies on the homologous recombination of...
Resource Type:: Dissertation
Full Text:: Creation of an efficient protein expression and secretion

Fatty acylation of Vaccinia virus proteins : dual myristylation and palmitylation of the A-type inclusion protein

Creator:: Martin, Karen H.
Abstract:: The attachment of myristic acid to the N-terminal glycine residue of many eukaryotic and viral proteins is often essential for the acquisition of the protein's biological activity. Vaccinia virus (VV), the prototype member of the Poxviridae, expresses several myristylated proteins during the course of infection. Only one of these proteins,...
Resource Type:: Dissertation
Full Text:: Acylation of Vaccinia Virus Proteins: Dual Myristylation and Palmitylation of the A-type Inclusion Protein

Genome-enabled discovery and characterization of type III effector-encoding genes of plant symbiotic bacteria

Creator:: Kimbrel, Jeffrey A.
Abstract:: Symbiosis is the close and protracted interaction between organisms. The molecular interactions that occur during symbiosis are complex with multiple barriers that must be overcome. Many Gram-negative, host-associated bacteria use a type III secretion system to mediate associations with their eukaryotic hosts. This secretion system is a specialized apparatus for...
Resource Type:: Dissertation
Full Text:: Appendix IV, Figure 2. Identification of expressed protein-coding genes as a function of sequencing depth

Identification and characterization of type III effector proteins in plant-associated bacteria

Creator:: Thomas, William J.
Abstract:: Symbioses between microbes and multicellular eukaryotes are found in all biomes, and encompass a spectrum of symbiotic lifestyles that includes parasitism and disease, commensalism, and mutually beneficial interdependent host-microbe relationships. Regardless of outcome, these symbiotic lifestyles are governed by a complex molecular "courtship" between microbe and potential host. This courtship...
Resource Type:: Dissertation
Full Text:: NADPH oxidase, calmodulin-mediated production of nitric oxide (NO), and a calcium-dependent protein

In vitro and in vivo approaches for the functional characterization of the scaffold protein, GRASP

Creator:: Venkataraman, Anand
Abstract:: Studies using the pluripotent embryonic carcinoma cell line, P19, as a retinoic acid (RA)-responsive model system have been instrumental towards our understanding of the RA-dependent signaling pathways in development and homeostasis. Grp1-associated scaffold protein (GRASP; also known as Tamalin) was first identified by our group, as a gene robustly induced...
Resource Type:: Dissertation
Full Text:: Approaches for Functional Characterization of the Scaffold Protein, GRASP. Abstract approved

Investigating Protein-lipid-membrane Interactions in Plant Cells using Bimolecular Fluorescence Complementation

Creator:: Tao, Kai
Abstract:: The overall focus of this thesis is on the distribution of specific lipids and membrane proteins of the external and internal membranes of plant cells, in the context of the roles that those lipids and proteins may play in microbe-plant interactions. The work includes the development of several new tools,...
Resource Type:: Dissertation
Full Text:: : Investigating Protein-lipid-membrane Interactions in Plant Cells using Bimolecular Fluorescence

Mismatch repair in plants : identification and characterization of Arabidopsis thaliana MutS homolog proteins

Creator:: Culligan, Kevin M.
Resource Type:: Dissertation
Full Text:: the eubacterial DNA- mismatch-repair protein MutS. In comparison to other eukaryotic organisms

ScholarsArchive@OSU

An emerging role for the exocyst : plant morphogenesis

Binding and assembly of H5 (and the globular domain of H5) onto DNA

Biochemical characterization and mutational analysis of human uracil-DNA glycosylase

Creation of an efficient protein expression and secretion system in the gram-positive bacterial vector, Streptococcus gordonii

Fatty acylation of Vaccinia virus proteins : dual myristylation and palmitylation of the A-type inclusion protein

Genome-enabled discovery and characterization of type III effector-encoding genes of plant symbiotic bacteria

Identification and characterization of type III effector proteins in plant-associated bacteria

In vitro and in vivo approaches for the functional characterization of the scaffold protein, GRASP

Investigating Protein-lipid-membrane Interactions in Plant Cells using Bimolecular Fluorescence Complementation

Mismatch repair in plants : identification and characterization of Arabidopsis thaliana MutS homolog proteins

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