The lack of a suitable demembranated cell model has hindered the
study of the mechanisms responsible for the regulation of ciliary
waveform and coordination. In this study procedures for the
isolation of ciliated cells from the newt, Taricha granulosa, by
trypsin dissociation, and their subsequent demembranation with Triton
X-100 and...
A method for isolating the salt gland (SG) from the larval brine
shrimp has been developed. This extra-renal organ is removed from 5-10%
of Stage Inauplii by vortexing after incubating the animals at 37°C for
8 hours in a saline solution buffered with 100 mM sodium phosphate at pH
7.6....
The benefits of reproduction are clear, but there are also costs. Much is known about the costs of reproduction in females, but only recently have male costs been investigated in any depth. These costs of reproduction may be minimized by appropriately modifying behavior, but there has been little research on...
Pheromones are chemical cues produced by organisms that affect the behavior and/or physiology of conspecifics. The orchestration of reproductive behaviors in many animals depends on the expression of sex pheromones. In insects, intraspecific variation in sex pheromone expression is commonly observed and often influences social interactions between individuals. To what...
Parasitic infections and immune challenges can affect host reproductive fitness and, ultimately, the evolution of host populations in a myriad of ways. The fitness implications of parasitic infections range from increased host mortality to subtle changes in reproductive investment. From alterations of behaviors, sexual signaling, and competitive ability to changes...
During 1966, 1967, and 1968 extensive field investigations were
carried out in Mount Rainier National Park. These included continuous
research during the summer months, and weekend activities
during the winter months. Small mammals were trapped and
observed, and the larger mammals were observed exclusively. The
summer trapping consisted of two...
Chromosome maps were made of Drosophila gibberosa salivary gland
and fat body chromosomes, and the puffing patterns were compared in
the two tissues for selected times in development. The times were
early third instar (E), late third instar (0, at pupariation
(0 hr), at two hours (2 hr), four hours...
In Artemia salina nauplii acclimated in 0.25 M, 0.5 M, 1.0 M, 1.5 M,
2.0 M, and 2.5 M NaCl incorporation of ¹⁴CO₂ into free amino acids was
greatly stimulated in the higher salinities. The ¹⁴C label was found in
aspartic acid, serine, glutamic acid, proline and alanine.
Hypersalinity also...
Dipterans of the family Ephydridae (shore and brine flies) are well
known for their ability to tolerate and thrive in a wide variety of
physically harsh circumstances. The immature stages of one such species
of this family, Ephydra hians, are characteristically limited in
distribution as benthic inhabitants of highly alkaline...