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Filtering by: Creator Simmons, Ryan S. Remove constraint Creator: Simmons, Ryan S. Degree Level Bachelor's Remove constraint Degree Level: Bachelor's

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  • Are they what they eat? A stable GIT microbiome characterized in P. resecata

    Creator:
    Gustafson, Tarah N.
    Abstract:
    Intertidal herbivores, such as isopods, help regulate and contribute to nutrient cycling and organic carbon flow through the trophic levels in estuaries and coastal ecosystems. Though much is known about the microbiomes of macrophyte leaves that serve as the primary food source for isopods, and (to a lesser extent) the...
    Resource Type:
    Honors College Thesis
    Full Text:
    :_____________________________________________________ Ryan Mueller Intertidal herbivores, such as isopods, help regulate and contribute to

  • Numerical Effects on Timing System Pathways

    Creator:
    Steiner, Samantha K.
    Abstract:
    Studying human’s ability to estimate temporal durations could lead to a greater understanding of our cognitive abilities as well as the processes that underlie temporal estimation. It has been shown through previous research that time and number may be interconnected cognitively and that the magnitude of one may alter the...
    Resource Type:
    Honors College Thesis
    Full Text:
    Dr. Lawrence Ryan _____

  • Site-Specific Modification of Glucose Proteins Enabling Precise Surface Orientation For Glucose Sensors

    Creator:
    Wolfenden, Nurhayati
    Abstract:
    An ideal labeling reaction between protein and surface should generate a protein-patterned surface with control over the orientation of the desired protein. Controlling orientation would generate a homogeneous, un-fouled protein surface and allow for maximum efficiency in regards to the activity, reactivity, and stability of the protein. In this research...
    Resource Type:
    Honors College Thesis
    Full Text:
    : ______________________________________________________ Ryan Mehl An ideal labeling reaction between protein and surface should generate a

  • Evaluation of total hip arthroplasty research in the US and Europe

    Creator:
    Simmons, Marcy J.
    Abstract:
    Background: Hip replacements are considered routine operations which improve the quality of life in patients by reducing pain and restoring function. There are many variations in prostheses with varying benefits and disadvantages. Methods: Literature from the United States and Europe was collected on total hip replacements between 2001 and the...
    Resource Type:
    Research Paper
    Full Text:
    Simmons 1 Evaluation of Total Hip Arthroplasty Research in the US and Europe by Marcy J

  • Engineering bioorthogonal reactions on proteins in live cells

    Creator:
    Machovina, Melodie M. and Mehl, Ryan A.
    Abstract:
    This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
    Resource Type:
    Poster

  • Engineering bioorthogonal reactions on proteins in live cells

    Creator:
    Machovina, Melodie M. and Mehl, Ryan A.
    Abstract:
    This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
    Resource Type:
    Poster

  • Engineering Oriented Protein-Labelled Surfaces

    Creator:
    Brown, Wesley and Mehl, Ryan
    Abstract:
    Current protein immobilization techniques lack defined control over protein orientation. Protein orientation is important for making biosensors and biomaterials that are sensitive and efficient and can be crucial for designing some devices such as immunosensors or direct electron transferring biocells. A new method of protein immobilization is proposed that takes...
    Resource Type:
    Presentation
    Full Text:
    Oriented Protein-Labelled Surfaces Wes Brown 1, Ryan Mehl PhD 2- Bioresource Research 1, Department of

  • Selection of an Orthogonal tRNA Synthetase for Trifluoromethyl-phenylalanine in Methanomethylophilus Alvus System

    Creator:
    Eyberg, Daniel R.
    Abstract:
    Genetic code expansion is a technology that allows for site-specific incorporation of noncanonical amino acids into recombinant proteins at amber stop codons using engineered translational machinery. The technology allows for expanded study into proteins, derived from the integration of novel chemical functions found in noncanonical amino acids, such as fluorescence,...
    Resource Type:
    Honors College Thesis
    Full Text:
    approved: _____________________________________________________ Ryan A. Mehl Genetic code

  • Controlled Orientation of Engineered Carbonic Anhydrase on a Surface : A Model for Protein Immobilization for Biosensors and Biomaterials

    Creator:
    Brown, Wesley
    Abstract:
    Current protein immobilization techniques lack defined control over protein orientation. Protein orientation is important for making biosensors and biomaterials that are sensitive and efficient and can be crucial for designing some devices such as immunosensors or direct electron transferring biocells. A new method of protein immobilization is proposed that takes...
    Resource Type:
    Honors College Thesis
    Full Text:
    : ______________________________________________________ Ryan A. Mehl ABSTRACT: Current protein immobilization techniques lack defined control

  • Characterizing the Interactome of Nitrotyrosine-Calmodulin using Genetic Code Expansion

    Creator:
    Kong, Garth L.
    Abstract:
    A specific tyrosine post-translational modification, called 3-nitro-tyrosine (nitroTyr), has been known to be present on an essential calcium signaling protein called calmodulin (CaM) during oxidative stress. While protein-bound 3-nitro-tyrosine has long been considered a biomarker of OS, it is also hypothesized to be a mechanism for protein signaling. However, purification...
    Resource Type:
    Honors College Thesis
    Full Text:
    : _____________________________________________________ Ryan Mehl A specific tyrosine post-translational modification, called 3-nitro