The association of other materials with DNA has been studied
extensively in higher organisms and more recently in bacteria.
However, investigation of these complexes in bacteria have for the
most part been restricted to the study of a specific function this association
may serve, or a specific kind of association....
A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl
chloride (dansyl chloride), was covalently bonded to polylysine. The
dansyl tagged polylysine was complexed to DNA by gradient dialysis.
The polarization and intensity of fluorescence of the dansyl and the
turbidity of the complex were measured as a function of salt concentration
for a variety of...
The genome of the nuclear polyhedrosis virus which infects the
larvae of the tussock moth, the lepidopteran, Hemerocampa
pseudotsugata, was isolated, identified as deoxyribonucleic acid, and
characterized in terms of its physical and biological properties. An
isolation procedure for the viral DNA was established which consisted
of the following steps:...
The DNAs (deoxyribonucleic acids)
of the thoracic tissue
of
the tobacco hornworm,
Manducta sexta, were studied
by physical
and chemical means. The adult
moth was considered to
be fully
formed two days after emergence from
the pupal case, as indicated
by its ability to fly and the integrity
of the...
The interaction of polylysine with DNA may be divided into two
separate phenomena: the primary interaction involved in formation of
a well-defined complex, and secondary interactions leading to aggregation
of these complexes. Most studies on the interaction of polylysine
with DNA have used techniques which measure properties of
the aggregated...
Using thermal denaturation methodology, the nucleic acid
base composition of deoxyribonucleic acid (DNA) isolated from type,
neotype and stock strains of Lactobacillus species was determined.
The genus was found to be heterogeneous in base composition,
ranging from 33.1 to 50.0 moles % guanine plus cytosine (GC).
Genus members fell into...
The genome composition and DNA base sequence homologies
of several diverse species of fish were examined. The fishes were
characterized from spectrophotometric studies of reassociation and
hybridization of their DNAs and from the thermal stabilities of hybrid
duplexes.
Spectrophotometric studies indicated that repeated sequences
comprise 35-40% of the genomes of...