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Physical studies of the yeast inner histones

Creator:: Mardian, James Kenneth Walker
Abstract:: In this work, the four yeast inner histones were prepared by the use of consecutive slab gel fractionation. The use of this technique allowed the first complete fractionation and rigorous identification of the four yeast inner histones, and made possible a study of their complexing behavior. Variants and adjuncts of...
Resource Type:: Dissertation
Full Text:: addition of salt, there is an immediate change in the circular dichroism and fluorescence anisotropy, but

Physico-chemical studies on the structure of eukaryotic chromatin

Creator:: Sahasrabuddhe, Chintaman Gopal
Abstract:: It has been shown that about 50 % of the whole chromatin DNA exists in a very compact form in calfthymus chromatin.These compact fragments can be extracted from the whole chromatin as nuclease resistant fragments by limited nuclease digestion. These fragments involve four out of five histones in folding the...
Resource Type:: Dissertation
Full Text:: for their support through the Grant No 37307 X. INTRODUCTION MATERIALS AND METHODS Preparation of

Physical properties of calf thymus H1 histones : conformational changes and interactions with two nonhistone chromosomal proteins

Creator:: Smerdon, Michael John
Abstract:: This work represents the first study of the conformational changes in histone H1 subfractions as well as the interactions of these subfractions with two nonhistone chromosomal (NHC) proteins. Calf thymus Hi was fractionated by the method of Kincade and Cole (1966a) using a very shallow guanidinium chloride gradient. A possible...
Resource Type:: Dissertation
Full Text:: by measuring the fluorescence anisotropy of the tyrosine emission, the circular dichroism (CD) of

Towards the understanding of the function of the histone "tails" with respect to the structure, stability, and function of chromatin

Creator:: Dong, Feng
Abstract:: By using immobilized trypsin, I have been able to prepare well-defined, stable trypsinized nucleosomes. The difficulties of lacking of control in the extent of trypsinization, which were encountered in previous studies with the use of free trypsin, have been eliminated. The nucleosomes and oligonucleosomes prepared by immobilized trypsin are suitable...
Resource Type:: Dissertation
Full Text:: function of the ionic strength 68 11-8 Circular dichroism spectra of the different nucleosome core

The binding of ethidium bromide to chromatin : model for carcinogen interactions

Creator:: McMurray, Cynthia T.
Abstract:: Five forms of chromatin, which represent the in vivo folded forms of nucleic acid, were isolated and used as the binding substrate for model intercalating compound, ethidium bromide. For all forms of chromatin, the affinity, location and structural effects of binding were examined. On the level of the nucleosome, the...
Resource Type:: Dissertation
Full Text:: to gain structural information about the native nucleoprotein complexes. The Binding of Ethidium

Binding and assembly of H5 (and the globular domain of H5) onto DNA

Creator:: Carter, George John
Abstract:: In order to better characterize linker histone interactions with DNA, avian erythrocyte-specific linker H5 and the trypsin-resistant globular domain of H5 (GH5) were used in DNA binding studies. To begin, H5 displayed a considerably higher binding affinity for DNA than the isolated globular domain (GH5), supporting the importance of the...
Resource Type:: Dissertation
Full Text:: 53 2.3 Circular dichroism of linker histone proteins, and the effect of PO4 3 55 2.4 Salt

Structure and function of chromatin : studies at the nucleosome and nuclear matrix levels

Creator:: Shih, Ah Shing
Abstract:: Several levels of eukaryotic chromatin structure have been observed: the nucleosome, the 10 nm and 30 nm fibers and loop domains, apparently attached to the nuclear matrix. In this research, the structure and function of chromatin at two of these levels was investigated, with studies on both nucleosome positioning and...
Resource Type:: Dissertation
Full Text:: facts and artifacts of our information about the structure and function of the nuclear matrix. I also

Effects of nucleosomes on transcription by polymerase I in a reconstituted system

Creator:: Georgel, Philippe, 1961-
Abstract:: The aim of this study was to gain more information about the interactions between DNA and the histone octamer during the process of transcription. This work used a pUC8 plasmid derivative that contained the core promoter region of the RNA polymerase I of Acanthamoeba castellanii, placed upstream of four repeats...
Resource Type:: Dissertation
Full Text:: : Restriction digestion of circular pPol I 208-4 plasmid DNA 47 2. 5: Restriction digestion of pPol I 208-4

On the location of the linker histones and the linker DNA in the 30 nm fiber of chromatin

Creator:: Leuba, Sanford H.
Abstract:: Understanding the structure of the 30 nm fiber in chromatin is relevant to understanding eukaryotic replication and transcription. The major controversy among the models of the fiber concerns the disposition of the linker DNA, the DNA between adjacent nucleosomes, and the location of the linker histones. To determine if the...
Resource Type:: Dissertation
Full Text:: coiled as continuation of nucleosome DNA coil 1.3 Helix of zigzag chain; no linker "crossover" 1.4

Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy

Creator:: Brown, D. W. (David W.), 1937-
Abstract:: Time-resolved fluorescence spectroscopy was used to monitor the effects of varying ionic strength on nucleosome core particle structure. Two main methods were used in these studies. First, the fluorescence anisotropy decay of bound ethidium was measured and was shown to reflect the rotational tumbling of the core particle through solution,...
Resource Type:: Dissertation
Full Text:: in the N-terminal domains. They found no evidence for crosslinking via the central regions of the

ScholarsArchive@OSU

Physical studies of the yeast inner histones

Physico-chemical studies on the structure of eukaryotic chromatin

Physical properties of calf thymus H1 histones : conformational changes and interactions with two nonhistone chromosomal proteins

Towards the understanding of the function of the histone "tails" with respect to the structure, stability, and function of chromatin

The binding of ethidium bromide to chromatin : model for carcinogen interactions

Binding and assembly of H5 (and the globular domain of H5) onto DNA

Structure and function of chromatin : studies at the nucleosome and nuclear matrix levels

Effects of nucleosomes on transcription by polymerase I in a reconstituted system

On the location of the linker histones and the linker DNA in the 30 nm fiber of chromatin

Conformational transitions of nucleosome core particles monitored with time-resolved fluorescence spectroscopy

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