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Properties and conformation of P-form DNA

Creator:: Zehfus, Micheal H.
Abstract:: Work is presented which explores the properties and nature of the P-form of DNA. Initially the P-form represented an unknown state of DNA which occurred when this molecule was in a solvent of 47.5% methanol, 5% water, and 47.5% ethanol, and which showed properties of both native and denatured conformations....
Resource Type:: Dissertation
Full Text:: circular dichroism spectrum of the P-form into the far UV where its low intensity in- dicates a lack of

A partial characterization of the deoxyribonucleic acid found in the thoracic muscles of the tobacco hornworm moth (Manducta sexta)

Creator:: Hinds, Thomas Richard, 1944-
Abstract:: The DNAs (deoxyribonucleic acids) of the thoracic tissue of the tobacco hornworm, Manducta sexta, were studied by physical and chemical means. The adult moth was considered to be fully formed two days after emergence from the pupal case, as indicated by its ability to fly and the integrity of the...
Resource Type:: Dissertation
Full Text:: 2) from contour lengths, measured on electron micrographs, of the circular and super coil forms

Isolation and characteristics of the DNA from nuclear polyhedrosis virus

Creator:: Carnegie, John William
Abstract:: The genome of the nuclear polyhedrosis virus which infects the larvae of the tussock moth, the lepidopteran, Hemerocampa pseudotsugata, was isolated, identified as deoxyribonucleic acid, and characterized in terms of its physical and biological properties. An isolation procedure for the viral DNA was established which consisted of the following steps:...
Resource Type:: Dissertation
Full Text:: suggested from the electronmicro- graphs that the viral DNA has a circular structure. Whereas all of these

Studies on the interaction of polylysine with deoxyribonucleic acid

Creator:: McKenzie, Raymond Leslie
Abstract:: The interaction of polylysine with DNA may be divided into two separate phenomena: the primary interaction involved in formation of a well-defined complex, and secondary interactions leading to aggregation of these complexes. Most studies on the interaction of polylysine with DNA have used techniques which measure properties of the aggregated...
Resource Type:: Dissertation
Full Text:: form. 7 Olins (55) finds no change in the circular dichroism of DNA when it is complexed with the

Effects of nucleosomes on transcription by polymerase I in a reconstituted system

Creator:: Georgel, Philippe, 1961-
Abstract:: The aim of this study was to gain more information about the interactions between DNA and the histone octamer during the process of transcription. This work used a pUC8 plasmid derivative that contained the core promoter region of the RNA polymerase I of Acanthamoeba castellanii, placed upstream of four repeats...
Resource Type:: Dissertation
Full Text:: : Restriction digestion of circular pPol I 208-4 plasmid DNA 47 2. 5: Restriction digestion of pPol I 208-4

Hydrodynamic properties of homogeneous double-stranded DNA

Creator:: Kovacic, Roger Timothy
Resource Type:: Dissertation
Full Text:: bacteriophage is circular and superhelical. Therefore this DNA contains no single-stranded breaks. Although

Crystal structures of DNA four-way junctions

Creator:: Eichman, Brandt F.
Abstract:: DNA four-way junctions (also known as Holliday junctions) are the primary structural intermediate during recombination, an important process responsible for biological evolution and maintenance of genomes. These junctions arise from the assembly of four nucleic acid strands to produce double-helical regions extending from a central point. Although much progress has...
Resource Type:: Dissertation
Full Text:: micrographs of plasmid DNA connected by four way junctions, showing figure-8 structures of closed circular

Studies on the hydrophobic effect and its contribution to the stability, crystallization, and helix packing of Z-DNA

Creator:: Kagawa, Todd F.
Abstract:: The studies presented here use the B- to Z-DNA transition and Z-DNA crystallization as model systems to determine the contribution of solvent interactions to macromolecular structures. The substituent groups of naturally occurring and modified nucleotide bases affect the hydration and thus the stability of right-handed B-DNA and left-handed Z-DNA. The...
Resource Type:: Dissertation
Full Text:: processes often depends on information concerning the structure and dynamics of macromolecules and their

The bacteriophage P1 bof protein : a corepressor whose action can both negatively and positively affect phage gene expression

Creator:: Schaefer, Timothy S.
Resource Type:: Dissertation
Full Text:: I.1 Circular map of bacteriophage P1 15 1.2 Map of P1 immunity regions 16 1.3 DNA sequences of P1

Site-specific aflatoxin B₁ adduction of sequence-positioned nucleosome core particles

Creator:: Moyer, Richard A.
Abstract:: The question of how the presence of nucleosomal packing of DNA modifies carcinogen interaction at specific sites cannot be answered by studies on whole chromatin or bulk nucleosomes because of the heterogeneity of DNA sequences in the particles. This problem was circumvented by constructing nucleosomes that are homogenous in DNA-histone...
Resource Type:: Dissertation
Full Text:: fraction of circular, but no linear plasmid. F. PREPARATION OF END LABELED 5S DNA FRAGMENTS Plasmid

ScholarsArchive@OSU

Properties and conformation of P-form DNA

A partial characterization of the deoxyribonucleic acid found in the thoracic muscles of the tobacco hornworm moth (Manducta sexta)

Isolation and characteristics of the DNA from nuclear polyhedrosis virus

Studies on the interaction of polylysine with deoxyribonucleic acid

Effects of nucleosomes on transcription by polymerase I in a reconstituted system

Hydrodynamic properties of homogeneous double-stranded DNA

Crystal structures of DNA four-way junctions

Studies on the hydrophobic effect and its contribution to the stability, crystallization, and helix packing of Z-DNA

The bacteriophage P1 bof protein : a corepressor whose action can both negatively and positively affect phage gene expression

Site-specific aflatoxin B₁ adduction of sequence-positioned nucleosome core particles

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