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Filtering by: Alternative title ssim EC 1141 Degree Level Doctoral Subject Escherichia coli -- Genetics

Characterization of Escherichia coli double-strand uracil-DNA glycosylase and analysis of uracil-initiated base excision DNA repair

Creator:

Sung, Jung-Suk

Abstract:

Escherichia coli double-strand uracil-DNA glycosylase (Dug) was purified to apparent homogeneity from bacteria that were defective in uracil-DNA glycosylase (Ung). After cloning the dug gene, recombinant Dug was overexpressed, purified, and characterized with respect to activity, substrate specificity, product DNA binding, and mechanism of action. Purified Dug excised both uracil...

Resource Type:

Dissertation

Full Text:

....................................................................................................... 233 5.1.1 Comparison of Substrate Specificity of Dug on U/G and eC/G DNA
Characterization of the Escherichia coli uracil-DNA glycosylase- inhibitor protein interaction

Creator:

Bennett, Samuel E.

Resource Type:

Dissertation

Full Text:

E. coli deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase, EC 3.6.1.23) plays a central role