A modified agar overlay technique for the preliminary screening
of antibiotic producing Streptomyces spp. has been developed and
compared to the routinely used shake flask method and the
unmodified agar overlay technique. The percentage of isolates
exhibiting antimicrobial activities was found to be greater in the
modified overlay technique.
Three...
This investigation was undertaken to provide a chemical
description of an actinophage for Streptomyces griseus, and to
determine the base composition of the phage DNA moiety. It was
believed of interest to learn whether the actinophage for the
filamentous S. griseus host would be different in its chemical
properties from...
Avermectins are a group of potent anthelmintic agents produced by Streptomyces avermitilis. They are active at extremely low dosages and are now in commercial use. In this study, the synthesis of two segments of avermectin B[subscript 1a] are described. The synthesis of the hexahydrobenzofuran subunit (segment A) began with the...
Glucose metabolism of phage-infected Streptomyces griseus
was examined by using the specific radioactivities of the amino
acids from the extracted protein of the host and phage as an index
of the catabolic pathways of glucose metabolism. Conventional
methods for the study of phage-infected bacteria did not suit the
host-actinophage system...
Early studies on the biosynthesis of the quinoline quinone portion of
streptonigrin, 1, had suggested the intermediacy of novel aromatic amino
acids. In order to test this hypothesis, a series of compounds was synthesized
and tested for potential incorporation. [4-¹⁵N] 4-Aminoanthranilic acid,
4 5 a , [4-² H] 7-aminoquinaldinic acid,...