Otoferlin is a C2-domain, transmembrane protein whose mutated form is responsible for the DFNB9 subtype of prelingual hearing impairment. Mutations in otoferlin cause an autosomal recessive, non-syndromic form of deafness that is characterized by auditory neuropathy/ auditory dissynchrony (AN/AD), meaning that the outer hair cells (OHCs) are normal, and the...
The focus of this dissertation was to understand protein function and structure ona molecular level. To do this successfully, a variety of biochemical and biophysicaltechniques were employed. A wide variety of techniques were applied to study theproteins of interest in this dissertation to probe protein-protein interactions, proteinfunction, protein structure, and...
A fundamental difference between prokaryotic and eukaryotic cells is the presence of membrane bound organelles in eukaryotes. The dynamics of membrane trafficking within the cell are responsible for everything from intercellular communication and cell homeostasis, to mitosis, cell migration, and differentiation. These processes require exocytosis and compensatory endocytosis, often working...
Hearing loss is one of the most common defect, affecting 360 million people worldwide due to several factors including congenital, present at or soon after birth or acquired with age. Congenital hearing loss affects 32 million children in the world. The economic impact of hearing loss is estimated to cost...
Dysferlin is a ∼230 kDa terminally anchored membrane protein that is ubiquitously expressed, but is particularly enriched in skeletal and cardiac muscle tissue. Mutations covering the length of the protein have been linked to muscle wasting diseases including limb-girdle muscular dystrophy and Myoshi myopathy. Dysferlin has been shown to play...
Auditory defects and disorders are prevalent at all ages and affect 8% of the population in developed nations including newborns and children. Congenital hearing loss is the most common birth defect and it is estimated that 1 in 1000 children are affected by deafness at birth or before the onset...
Congenital hearing loss is a common disorder and over 60 mutations in the sensory hair cell protein, otoferlin, have been linked to pre-lingual deafness. Although otoferlin is essential for hearing, the large size and low solubility of the protein have limited approaches to study its function on a molecular-level. To...
Peroxiredoxin (Prx) enzymes catalyze the reduction of hydrogen peroxide, peroxynitrous acid, and organic peroxides, and are extremely efficient peroxidases, with k[subscript cat]/K[subscript M] on the order of 10⁷ - 10⁸ M⁻¹ s⁻¹. Besides their role in oxidative stress defense, evidence has accumulated that some eukaryotes, including humans, use Prxs as...
The purpose of this study was to investigate the initial on-site supervision experience of school counseling interns. Current counseling supervision research has failed to address dynamics specific to the context of school counselor training and professional development. This gap in the research suggest that examining the phenomenological experience of what...
In this dissertation, I describe spectroscopic studies of jet-cooled polycyclic aromatic hydrocarbons (PAH) and one nitrogen substituted PAH (PANH) using pulsed field zero kinetic energy (ZEKE) photoelectron spectroscopy and resonantly enhanced multiphoton ionization (REMPI) spectroscopy. Recently, there has been a demand for far-infrared (FIR) spectral information of astrophysically relevant molecules...
The goal of this dissertation was to improve our abilities in acquiring critical in vivo data by establishing embryo-larva zebrafish as an exemplary model to quantitatively evaluate cancer progression. The living environment that encompasses a tumor has a significant effect on how cancer develops, grows and metastasizes. In order to...
Work is presented which explores the properties and nature of
the P-form of DNA. Initially the P-form represented an unknown
state of DNA which occurred when this molecule was in a solvent of
47.5% methanol, 5% water, and 47.5% ethanol, and which showed properties
of both native and denatured conformations....
The overall focus of this thesis is on the distribution of specific lipids and membrane proteins of the external and internal membranes of plant cells, in the context of the roles that those lipids and proteins may play in microbe-plant interactions. The work includes the development of several new tools,...
Intrinsically disordered proteins (IDPs), protein regions (IDRs), and protein complexes continue to emerge at the forefront of protein science. Proteins and protein regions lacking specific structure are found in all organisms, and often have vital roles in numerous biological processes. Breaking the well-known structure-function paradigm, the understanding of disorder-based functionality...
The Hippo signaling pathway is an evolutionarily conserved regulator of cell growth, proliferation, and apoptosis. A key function of the pathway is to regulate the subcellular distribution and activity of Yorkie in Drosophila or Yes-associated protein (YAP) and transcription co-activator with PDZ-binding motif (TAZ) in mammals. The dysregulation of the...
Alzheimer’s Disease (AD) is a neurodegenerative pathology currently affecting nearly 44 million individuals worldwide, yet there are not currently any effective treatments or preventions for AD despite the rapid development in our understanding of the disease over the last four decades. The medical and sanitary innovations of the last century...
Small molecule drugs have been instrumental in our fight against diseases, beginning when some of the earliest ancient cultures used plant remedies to treat illnesses. More recently, the skill of isolating and characterizing the active ingredients within bioactive plants and microbial extracts has led to the isolation and design of...
In Part I, (4S,5R)-4,5-dimethyl-4-phenylcyclohex-2-enone (19a) was prepared in 73% yield with high enantio- and diastereo-selectivity (er > 98:2, dr > 20:1) on a multigram scale by a Yamada-Otani condensation between (E)-pent-3-en-2-one and 2-phenylpropanal catalyzed by a sulfonimide derivative of (S)-proline (18, HuaCat®). Synthetically useful transformations of the cyclohexenone product 19a...
In this Dissertation, the mechanism and stereomechanics of complex organocatalysis and reaction processes are disclosed. The technologies and protocols currently being developed and utilized to efficiently investigate these systems using theory are highlighted.
A detailed computational study of peptide-catalyzed acylations is disclosed. In particular, the magnitude of all possible non-bonding...
In this dissertation, excited state proton transfer (ESPT) and its inhibition in solution and protein environments are revealed using both femtosecond transient absorption (fs-TA) spectroscopy and femtosecond stimulated Raman spectroscopy (FSRS). Using a tunable Raman pump to enhance transient vibrational features of the photoacidic chromophore HPTS in methanol and methanol...
The inverse electron demand Diels Alder reaction between tetrazines and strained alkenes is an exceptionally useful tool in functionalizing to biomolecules since it is orthogonal to the chemistry of most living systems and have exceptionally high rate constants. In particular reactions between strained trans-cyclooctenes (sTCO) and tetrazines can achieve second...
Higher education is being asked to address issues of quality, accountability, and transparency. Accreditation is one tool higher education institutions use to address these concerns. The purpose of this quantitative study was to analyze the impact of the new accreditation standards adopted by the Northwest Commission for Colleges and Universities...
In 2010 the American taxpayers spent $697 million educating veterans under the provisions of the Servicemen's Readjustment Act of 1944 (commonly referred to as the GI Bill). Most veterans begin their college education at a community college. However, the number of veterans succeeding in community colleges is exceedingly low. Based...
: ______________________________________________________
Earl P. Johnson
In 2010 the American taxpayers spent $697 million educating veterans under the
How do organizational members socially construct the shared meaning of a mandated change in the external environment? The opportunity to address this question presented itself when President Barack Obama set forth a goal for American community colleges to increase the number of students completing certificates and degrees by 5 million...
Background. As part of the nationwide student completion agenda, the Achieving the Dream (ATD) national reform network was established to help community colleges quickly institute sustainable change scaled to impact all students at the institution in a short period of time. Although ATD has been in existence for over a...
Institutions of higher education are increasingly being held accountable and experiencing pressure to demonstrate how well their students are achieving intended learning outcomes. External and internal influences fuel this pressure. After decades of rhetoric on student learning outcomes assessment there remains a question of whether the practice has been fully...
Studies of the use of NADH as the monitored fluorophore for enzymatic fluorescence kinetic methods are discussed. Interference studies
for the detection of NADH and the adaptation and optimization of fluorescence kinetic measurements to four serum assays are also described.
A microcomputer-controlled flow injection analysis (FIA) system was
designed and...
Recently researchers have discovered that groups of small non-coding RNAs (ncRNAs) play regulatory roles in gene expression and participate in various biological processes. For example, pathogenesis of many diseases, cell cycle regulation, and signaling pathways. Intracellular and live cell imaging of small ncRNA groups will reveal their relative expression levels...
The cellular dynamics of the intestinal epithelium in the coho
salmon, Oncorhynchus kisutch, has been studied to determine the effects of temperature and whole-body X-irradiation.
In vivo experiments utilizing colchicine mitotic inhibiting technique and tritiated
thymidine (³H-TdR) radioautography were employed in order to
evaluate the kinetics of the cell population....
This dissertation investigates the effect of endogenous and exogenous events on firm behavior and performance. These are fundamental questions in economics. The contribution of this study is threefold. First, it provides estimates of the impact of mergers on railroad efficiency, which has important antitrust implications. Second, it provides new estimates...
Preventative methods for software reverse engineering have been given greater attention in recent times due to the increase in computational resources and tools available to the public. The inherent security provided by encoding source into machine code (executable form) can no longer be assumed, given the availability of effective automated...
Given the many significant roles that bacteria play in modern food manufacturing, it is crucial that we have a fundamental understanding of how bacterial communities assemble and behave in the food manufacturing environment. The focus of this dissertation was to investigate both the spatial and temporal distributions of microbial communities...
Forests are highly valuable resources providing both timber as well as many non-use values like habitat for various endangered and threatened species, carbon sequestration, and recreation, to name a few. Given that there are 765 million acres of forestland in the United States, almost 60% of which are privately owned,...
Forest growth and yield models are critical to supporting decision making in forestry, but often lack considerations for wood properties. The feasibility of simulating wood properties in the context of a Douglas-fir individual tree growth and yield model was evaluated. This assessment explored the effect of predicted sapwood width, stem...
Just as the structure of a protein determines its function, protein motions and lack-of-structure are also key to regulating protein behavior. The human eye lens is made up of high concentrations of extremely stable crystallin proteins, but even in this context, small motions over time can cause a loss of...
Invasive plant species are expanding and transforming vegetative communities across Oregon and throughout the United States. Over the past three decades remote sensing, geographic information system (GIS), and Global Positioning System (GPS) technologies have been integrated to detect and map the distribution of noxious rangeland plants. This study developed low-cost...
The application of modern land management practices beginning at the turn of the 20th century is widely believed to have dramatically transformed forest landscapes of the inland Pacific Northwest. Restoring historical conditions to make forests resilient to future climate and disturbance regimes is a major goal of federal forest managers....
Research examining disparity between baccalaureate completion among "native" and community college transfer students has gained attention in recent years. Current and prior research has identified important pre-college and demographic characteristics with a positive relationship to baccalaureate completion. Yet few studies have focused on the influence of nonacademic and academic environmental...
Background. Many studies addressing the reasons why minority students fail in higher education are based on what is defined as a deficit model. A deficit model documents that a significant number of minority students fail because their culture is distinct from that of the majority population. Numerous minority students do...
The semiconductor gallium arsenide (GaAs) has many potential
advantages over the more widely used semiconductor silicon (Si).
These include higher low field mobility, semi-insulating substrates,
a direct band-gap, and greater radiation hardness. All these
advantages offer distinct opportunities for implementation of new
circuit functions or extension of the operating conditions...
This thesis details my investigation of some pharmaceuticals and natural products on the transport of drugs which are substrates of P-glycoprotein (P-gp), such as cyclosporin A (CSA) and digoxin by using Caco-2 and MDCKII-MDR1 drug transport models. Three objectives were performed to address this goal. The first objective was to...
Contracting nucleolar vacuoles are a general and consistent
feature of somatic tobacco cells growing in microcultures. Nucleolar
vacuoles were studied in cells from callus tissues of two
single-cell clones, H-196 and H-239, of hybrid tobacco (Nicotiana
tabacum X N. glutinosa). Vacuoles periodically contracted either
completely or incompletely, apparently releasing a...
Three highly purified forms of cytochrome P-450 (P-450a, P-450b, P-450c) from Aroclor 1254-induced rat hepatic microsomes catalyzed the monooxygenation of 1-(2- chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea (MeCCNU) and 1-(2-chloroethyl)-3-(cyclohexyl)-1-nitrosourea (CCNU) in the presence of purified NADPH cytochrome P-450 reductase, NADPH and oxygen. Cytochrome P -450- dependent monooxygenation of CCNU gave six alicyclic hydroxylation products...
The refresh times of all dynamic charge storage devices, best
characterized by the generation lifetime at roan temperature and the
recombination lifetime at higher device operating temperatures
(T>70°C), strongly influence the efficient and successful operation
of dynamic charge storage devices such as DRAM's and CCD's. Both
recombination and generation lifetime...
Winter squash (Cucurbita maxima) grown in Oregon’s Willamette Valley for edible seed, frozen foods, and fresh markets is susceptible to an undiagnosed soilborne disease. Diseased squash fields exhibit symptoms of stunting, root and crown rot, vascular discoloration, and late-season wilt, which in extreme cases can lead to total crop failure....
The objectives of this research were to 1) evaluate the establishment and peak standing crop of Berber Orchardgrass (Dactylis glomerata var. "Berber"), Palestine Orchardgrass (Dactylis glomerata var. "Palestine"), and Sheep Fescue (Festuca ovina var. "Covar") ; 2) to assess establishment and production changes resulting from environmental gradients (elevation, slope, aspect,...
The heritability of resistance to eastern filbert blight, caused by Anisogramma anomala, in European hazelnut (Corylus avellana) was evaluated in the progeny of seven cultivars crossed in 12 combinations. Progeny produced by crossing 'VR6-28' with three susceptible cultivars segregated 1:1 for complete resistance to eastern filbert blight. Histograms of disease...
The purpose of this research was to determine whether hybridization between Amelanchier denticulata. (H.B.K.) Koch and Amelanchier nervosa (Decaisne) StandL occurs in Oaxaca, Mexico, and to evaluate components of yield and germination characteristics and seed dormancy of Amelanchier denticulata. Amelanchier nervosa and Amelanchier denticulata samples and morphological characters were used...
Vacuum ultraviolet absorption spectra of 13 model sugar compounds
were measured in the energy range of 50 to 80 kK. The model
sugars included three simple alcohols, four simple ethers, four cyclic
alcohol-ether compounds, and two dioxanes. All spectra show
transition bands in the energy region 50 to 65 kK....
Deterministic source signature deconvolution is applied to the
processing of marine wide angle and vertical profiler data with air-gun
sources. Optimum results are obtained with a source signature
measured by stacking the signal reflected from a relatively homogeneous
abyssal plain sedimentary environment. This eliminates the need for
the unstable inverse...
Independent systems theory has been used to interpret the
circular dichroism (CD) spectra in the vacuum uv of three cyclic
dimers of amino acids. First, the theory for rotational strengths is
developed in terms of degenerate and nondegenerate group functions.
All terms to first order in perturbation theory are kept....
Seismic refraction, reflection and gravity data obtained
across the Peru continental margin and Nazca Plate
at 9° S. permit a detailed determination of crustal structure.
Complex structures normal to the profile require the
development of a ray trace technique to analyze first and
later arrivals for eleven overlapping refraction lines....
The main objective of this research was to assess the
nutritional status of sheep and goats grazing two rangeland
types in Baluchistan, Pakistan. These types were Artemisia
maritima/Haloxylon griffithii association represented by the
Zarchi Field Station and Cymbopogon shoenanthus, found at
the Tomagh Field Station. Seasonal above ground biomass
production...
Job satisfaction has received considerable attention within the community college sector, but there is limited research focused solely on the job satisfaction of foreign-born faculty members employed at community colleges. The purpose of this study was to examine factors associated with job satisfaction among foreign-born faculty members employed at U.S....
The circular dichroism spectra of amylose, the maltose
oligomers, cellobiose, and the cycloamyloses are measured in
aqueous solution to 1640 A. Two bands are found in this region. The
long wavelength band is assigned to the ether chromophores; the short
wavelength band is assigned to the hydroxyls and further ether...
The association of hyaluronic acid (HA) into a double stranded
structure has been investigated by circular dichroism (CD)
spectroscopy into the vacuum ultraviolet region. The CD of HA changes
dramatically, monitoring a cooperative transition as the dielectric
constant of an aqueous solution is reduced by adding organic solvents.
This structural...
A sophisticated computer program is developed to analyze flow linear
dichroism data on nucleic acids for individual base inclinations. Measured
absorption and linear dichroism data for synthetic AT and GC polymers and
natural DNAs are analyzed. The reliability of the program is tested on data for
the synthetic polymers, and...
A simple matrix method for the analysis of protein circular dichroism (CD) spectra is presented and used to compute the secondary structure of globular proteins in their native and denatured states. The method uses singular value decomposition and generalized inverse algorithms, and a basis set of CD spectra for proteins...
Large dams and their respective reservoirs can provide renewable energy and water security, but also profoundly alter riverine ecosystems. In the Pacific Northwest, dams and reservoirs cause discontinuities in river networks that have been particularly problematic for anadromous fishes. As barriers to the upstream and downstream migration of anadromous fishes,...
Background. As community colleges are increasingly composed of Millennial-aged professionals, workforce retention of those professionals is now a major priority. The Millennial generation is more diverse and more likely to be impacted by technology than any generation before them and is a generation characterized by dual-income partnerships. These and other...
The complexity of forest management has increased with the scope of resources of concern and the level of scrutiny from stakeholders. The design and use of specialized computer software, often referred to as “decision support systems” (DSS), is one method for helping managers deal with this complexity. DSS have proven...
Shear wave attenuation measurements were made
using ceramic bimorph transducers to excite transverse
vibrations in a cylindrical column of unconsolidated
sediment. Three different water-saturated
sediments were used in an attempt to determine the
effects of grain shape and sorting on the frequency
dependence of attenuation. The mean grain size of...
Small herds of cows were observed and spatially mapped over continuous twenty-four hour periods. Treatments were implemented that investigated the effect of water site and supplementation on animal distribution patterns. A series of six observation periods constituted each observation series. Observation series were repeated winter (January) and summer (July) for...
Bedrock meandering rivers are sinuous channels that pair steep bedrock outside banks with lower gradient slip-off slopes on the inside of bends. These rivers have the potential to record information on climatic and tectonic “external” forcings in their morphology (e.g., longitudinal profiles, strath terraces, channel and valley dimensions). However, geology...
A biographical study of Denison Olmsted, focusing upon his
own Christian world view and its connection with his various
activities in science, supports the view that religion served as a
significant factor in the promotion of science in America during this
time period. Olmsted taught physics, meteorology and astronomy
at...
A method based on fatty acid (FA) analysis is used to profile microbial community structure (MCS). Various extraction protocols are available, which alter the types of FAs extracted from soils. The more time consuming but widely used protocol extracts only FAs from phospholipids (PLFA). This technique is desirable because PLFAs...
Muscle weakness and fatigue are debilitating symptoms of multiple sclerosis (MS). Approximately 50% of muscle weakness and fatigue have been attributed to deficits within the peripheral nervous system, specifically mechanisms residing in the muscle. The goals of this study were to identify the cellular mechanisms of contraction within the muscle...
BaCuChF (Ch = S, Se, Te) materials are chalcogen-based transparent conductors with wide optical band gaps (2.9 – 3.5 eV) and a high concentration of free holes (10¹⁸ – 10²⁰ cm⁻³) caused by the presence of copper vacancies. Chalcogen vacancies compensate copper vacancies in these materials, setting the Fermi level...
This thesis describes a Mossbauer effect and electron
paramagnetic resonance study of iron (III) tetraphenylporphyrinatobenzenethiolate
benzene thiol, an analog compound
for bacterial cytochrome P-450. The compound is unusual in
that it provides a model for the two ferric reaction states
of cytochrome P-450. The iron in the model compound exhibits...
An intermediate of oxidative phosphorylation was apparently
solubilized from cabbage mitochondria. The method of solubilization
used primarily was extraction of a mitochondrial acetone powder
with 0.1 M glycylglycine, pH 7.4, at 4° C. The intermediate
betrayed its presence by causing the transfer of Pi³² to ADP with
the resultant formation...
The purpose of this multi-case study was to explore the stories and lived experiences of Native American police chiefs who have faced the systemic conflicts and challenges created between Native American tribal policing and an opposing U.S. legal system. It sought to understand the essence of tribal police chiefs’ experiences...
This thesis studies connections between disorder type in tree polymers and the branching random walk and presents an application to swarm site-selection. Chapter two extends results on tree polymers in the infinite volume limit to critical strong disorder. Almost sure (a.s.) convergence in the infinite volume limit is obtained for...
Seismic refraction measurements along two unreversed lines
indicate that the earth's crust is 26 km thick in southeastern Alaska
and 30 km thick along the Inside Passage of British Columbia. The
crust in southeastern Alaska, north of Dixon Entrance, consists of
a layer 9 km thick with a seismic velocity...
Increasing demands on earth resources require the need to
know how much resource is present. A new technology, which is
developing partially with the support of the National Aeronautics and
Space Administration, will help to inventory and monitor surface resources
in a more comprehensive manner than previously possible.
Research in...
Using the Matsubara Green's function technique we calculate the
infrared polariton dispersion relation from the lowest order terms of
a Hamiltonian which includes radiation fields and electron effects.
The formalism allows generation of higher order interaction terms,
one of which contains the Frohlich conduction electron optical phonon
interaction. By comparing...
Karl Jordan (1861-1959) was an extraordinarily productive entomologist who influenced the development of systematics, entomology, and naturalists' theoretical framework as well as their practice. He has been a figure in existing accounts of the naturalist tradition between 1890 and 1940 that have defended the relative contribution of naturalists to the...
The complete genome of snakehead rhabdovirus (SHRV) was cloned and
molecularly characterized. This was initially accomplished through the sequence
determination of its glycoprotein gene and the phylogenetic analysis of this gene with
orthologous genes from other rhabdoviruses. The phylogenetic analysis revealed that
SHRV groups with viruses of the genus Novirhabdovirus....
In grasslands, grazing by large ungulates can influence vegetation structure, composition, primary productivity, and ultimately, ecosystem functioning. While grazing represents a complex disturbance, grazing intensity largely determines the effects of
grazing on vegetation. Structural and compositional changes in the plant community caused by grazing could have bottom-up effects on species...
The coronavirus pandemic and its associated public health response resulted in disruptions in daily life as well as financial and other insecurities. This corresponded with an increase in adverse mental health outcomes in adolescents in the United States, including a temporary increase in suicidal ideation and behaviors. The pandemic also...
The defining feature of our planet are the oceans, which make up 70% of the Earth’s surface. The importance of the ocean cannot be understated: 50% of oxygen originates from phytoplankton; heat is absorbed and redistributed by ocean currents; and hundreds of millions of people rely on the ocean for...
Traditional approaches to streaming H.264 video over a network typically rely on a single method of transport (i.e., reliable or unreliable) and/or use static values for parameters that can have a significant negative impact on the perceptual quality of the received video. This dissertation presents a dynamic method for wireless...
Load flow studies are one of the most essential engineering applications for grid operators because they provide a clear picture of the operating conditions of the power system. Traditionally, the load flow problem is assumed to be deterministic; that is, the system variables contain no randomness. Unfortunately, this assumption is...
Many species have complex life cycles in which a dispersive larval stage is
followed by a relatively sedentary adult stage. For such species, reproductive output
is often high and large variation in survivorship throughout early life-history phases
(eggs and larvae) can lead to dramatic fluctuations in recruitment which may in...
Title: African-American Women Faculty Teaching at Institutions of Higher Learning in the Pacific Northwest: Challenges, Dilemas, and Sustainability
Institutions of higher learning in the Pacific Northwest have successfully recruited African-American faculty, yet these institutions have difficulty retaining African-American faculty for at least five years. African-American women faculty experience problems obtaining...