Differential expression (DE) analysis is a key task in gene expression study, because it uncovers the association between expression levels of a gene and the covariates of interest. This dissertation pertains to two particular aspects of DE analysis—identifying stably expressed genes for count normalization and accounting for correlation between DE...
Differential expression (DE) analysis allows us to identify genes that respond differently under varying experimental conditions, therefore granting us an understanding of the molecular basis of phenotypic variation. Following the identification of significantly differentially expressed genes, it is often of the researcher's interest to elucidate hidden patterns or groups in...
RNA-Sequencing (RNA-Seq) has rapidly become the de facto technique in transcriptome studies. However, established statistical methods for analyzing experimental and observational microarray studies need to be revised or completely re-invented to accommodate RNA-Seq data's unique characteristics. In this dissertation, we focus on statistical analyses performed at two particular stages in...
The absorption, translocation and metabolism of a new
herbicide, n-propyl-C¹⁴ N, N-di-n-propyl-thiolcarbamate (PDPC), were
studied in both peanut and soybean plants. These studies were
carried out on plants of different ages and treated at two rates of
application. The residue content of the plants was also determined
from germination to...
Vibrio marinus, strain MP-1, was shown to grow from pH 6.7
to 8.5, with an optimum at 7.3. Growth was inhibited below salinity
10%₀ and above 60%₀ . The upper temperature limit of
growth was 20 C, and growth was very rapid with aeration at 15 C.
Heating of the...
Compounds belonging to the vitamin K family possess anti-hemorrhagic
property, and are used in treating patients suffering
from hypoprothrombinemia and obstructive jaundice. Some of
these compounds also exhibit marked antimicrobial activity toward
various microorganisms. Vitamin K₅, 4-amino-2-methyl-
1-naphthol hydrochloride, a water-soluble analog of vitamin K
has been shown to possess...
As modern systems continue to increase in size and complexity, they pose significant safety and risk management challenges. System engineers and much of the government research efforts are focused on understanding the attributes and characteristics that emerge from the interactions of components and subsystems. As a result, the objective of...
While many organizations claim to "put safety first," safety is rarely considered early in the design process when system-level architectural decisions are made. Instead, system design follows an abstraction-to-detail process to first meet functional and then performance requirements. Following this process, safety assurance occurs in the later stages of design...
The effects of various physical parameters on the binding, uptake,
and catabolism of glucose in the marine psychrophilic bacterium,
Vibrio marinus, were studied. It was shown that shortly after
the cell was exposed to labeled glucose, the radioactive label became
rapidly associated with the cell fraction. It was also shown...
A gap exists in the methods used in industry and available in academia that prevents customers and engineers from having a voice when considering engineering risk appetite in the dynamic shaping of early-phase conceptual design trade study outcomes. Current methods used in Collaborative Design Centers either collect risk information after...