Monoclonal antibodies were developed against serotype 3, P-1059 strain of Pasteurella multocida. Enzyme-linked immunosorbent assays (ELISA) were used to detect antibodies in supernatants of hybridoma cells to either a surface protective (2.5 S) antigen prepared from a 2.5% saline extract or lipopolysaccharide (LPS) prepared by the phenol-water method. Forty two...
Saudi Arabia is one of the most arid countries worldwide with limited surface water sources. In most areas of the country, there are no natural rivers or lakes and precipitation is unpredictable and low. The entire region is susceptible to climate change, especially considering that the country is experiencing unprecedented...
Novel mucosal vaccines (LL-M2e, LL-HAe, SG-HAe) were constructed from live, non-pathogenic Lactococcus lactis or Streptococcus gordonii that express conserved regions of HA or M2 antigens from avian influenza virus (AIV) A. All three vaccines evoked antigen-specific serum immunoglobulin G (IgG) responses in vaccinated chickens. The addition of the adjuvant cholera...
Pasteurella multocida causes diseases in many animal
species including fowl cholera, a septicemic disease of
poultry and other birds. Pathogenesis of the disease has
been studied by many investigators by the systemic
administration of the organism in poultry. However, only a
few studies have been done as to the respiratory...
Protective antigens were separated from a 2.5% saline extract of
Pasteurella multocida type 4 by chromatographic methods. The organism
was grown for 18 to 20 hours at 37°C on an agar medium containing 5%
heat-inactivated turkey serum. The bacterial growth was harvested in a
2.5% NaC1 solution and agitated for...
The ability to move towards favorable environmental conditions, called chemotaxis, is common among motile bacteria. In particular aerotaxis has been extensively studied in Escherichia coli. Three putative aer gene homologs were identified in the V. cholerae genome designated VCAer-1 (VC0512) VCAer-2 (VCA0658), and VCAer-3 (VCA0988). Deletion analyses indicated that only...
Initial sites of localization and multiplication of Pasteurellamultocida were examined in chickens intravenously inoculated with varying doses of bacteria. Sections of lung, liver, kidney, bone marrow and spleen were prepared for light microscopic examination.Tissue sections were stained with the hematoxylin and eosin stain,Gram's stain or the avidin-biotin-peroxidase complex immunoenzymatictechnique. Of...
The Boivin agent from Vibrio anguillarum was extracted with the use of 0.25M trichloroacetic acid (TCA). Chemical analysis revealed that the endotoxin contained approximately 35.1% protein, 18.1% carbohydrate, 39.3% lipid, 1.8% glucosamine and evidence of ethanolamine. Sixteen different amino acids made up the protein. The aliphatic amino acid concentration was...