Gadusol is a UV-B-absorbent compound found in fish and other marine organisms where it is presumed to play a role as a sunscreen and antioxidant. In light of commercial potential as a replacement for problematic synthetic sunscreens, a process to produce recombinant gadusol in the yeast Saccharomcyces cerevisiae was investigated....
Reductive sulfate assimilation, the biological process by which sulfur-containing amino acids and key derivatives are synthesized from sulfate, is broadly shared among bacteria, fungi, and plants. It is the major, if not sole source of methionine and cysteine for Saccharomyces cerevisiae during wine fermentation. Two obligate intermediates formed in the...
Sequence-specific PCR markers were derived from one new and eight previously identified random amplified polymorphic DNA (RAPD) markers for the purpose of identifying grape (Vitis) rootstocks. The markers were developed because the RAPD assay was found to be inconsistent and the original RAPD markers unreliable. Southern hybridization analysis of the...
Sulfite mutants representing five complementation groups, previously derived from
an ethyl methanesulfonate-treated haploid strain of Saccharomyces cerevisiae were studied.
Although the wildtype S. cerevisiae strain used (isogenic to X2180-1 A) had a basal
tolerance for sulfite (7 μM free H₂SO₃), the sensitive and resistant mutants were found to
tolerate less...
A number of methods to isolate and quantify protein in Pinot noir wines were evaluated. The combination of precipitation by acetone containing 10% w/v trichloroacetic acid followed by quantification with the Bradford assay, reported in yeast invertase equivalents yielded the most accurate results when compared to micro-Kjeldahl analysis. The technique...
Yeast mannoproteins released during the process of aging wine on the yeast lees have been reported to make important contributions to wine quality. However, few mannoproteins have been identified in wine and their lifespan during aging is unknown. As a first step towards better understanding the contributions of yeast mannoproteins...
1-Phenyl-3-(2-Thiazoly1)-2-Thiourea (PTTU), has been reported to
inhibit dopamine-fi-hydroxylase (a copper-containing enzyme involved in
the pathway of biosynthesis of adrenaline in animal systems). In the
present study, PTTU has been shown to have cytokinin activity in the
tobacco bioassay. The cytokinin activity of PTTU suggested that
cytokinin-active N⁶-substituted adenine derivatives might...
Cytokinins are plant hormones regulating cell division and differentiation.
Most developmental events from germination, vegetative morphogenesis to seed
and fruit set are influenced by cytokinins. t,ns-Zeatin is the major natural
occurring cytokinin. Its isomer, cis-zeatin, and its derivatives are usually present in
low quantities and have traditionally been considered as...
One of the most common problems in wine production is the formation of hydrogen sulfide (H₂S) and other volatile sulfur compounds (VSCs), which can mask positive aromas at low concentrations and result in unpleasant aromas such as rotten egg, cabbage, garlic, and rubber at higher concentrations. Despite the large body...
'Golden Delicious' winter squash (GDWS), Cucurbita maxima Duchesne, provides significant amounts of pollen (24 mg) and nectar (236 μl), but with a low reward of 14% nectar sugar. The quantity of nectar produced per GDWS flower differed between sites and floral sex. The GDWS male flowers had 25% higher sugar...