The Boivin agent from Vibrio anguillarum was extracted with the use of 0.25M trichloroacetic acid (TCA). Chemical analysis revealed that the endotoxin contained approximately 35.1% protein, 18.1% carbohydrate, 39.3% lipid, 1.8% glucosamine and evidence of ethanolamine. Sixteen different amino acids made up the protein. The aliphatic amino acid concentration was...
Endotoxin is a part of the cell wall of gram-negative
bacteria, consisting of serotype-specific polysaccharide,
core oligosaccharide, and lipid A. Lipid A is responsible
for an array of pathophysiologic reactions in animal hosts.
Amebocyte lysate originated from Limulus polyphemus
(horseshoe crab) has been used extensively in various assay
systems to...