Strawberry is one of four crops included in the USDA-NIFA Specialty Crop Research Initiative-funded RosBREED project along with apple, peach, and cherry. Phenotyping strawberry for specific horticultural and commercial traits is important to identify associations with genotypic marker(s). This process is the first step in translating genomic knowledge into enhanced...
In recent years, there has been renewed interest in black raspberry (Rubus occidentalis L.) breeding. This has been spurred by an increase in black raspberry consumption due to studies that have shown them to be particularly high in anthocyanin content indicating high levels of antioxidants. Present cultivars are ill adapted...
Nuclear DNA flow cytometry was used to differentiate ploidy level and determine nuclear DNA content in Rubus. Nuclei suspensions were prepared from leaf discs of young leaves following published protocols with modifications that included: increasing the stain concentration, adding the stain after the RN ase treatment instead of adding it...
Hereditary variation is a vital component in the development of new and improved cultivars of landscape plants. Sources of hereditary variation include naturally occurring variation, recombination due to controlled crosses, artificial mutagenesis, and genetic modification via biotechnology. Here I explore all methods with the exception of genetic modification via biotechnology....
Strawberry is economically the most important berry crop grown worldwide and breeders are continuously striving to develop improved cultivars. So far, marker assisted breeding (MAB) in strawberry has been limited to the private sector. However, loci controlling some traits of economic importance such as perpetual flowering (PF) and soluble solids...
Pre-existing Expressed Sequence Tag (EST) libraries (1,305 sequences) and a newly constructed microsatellite-enriched genomic library (136 clones) from the V. corymbosum cultivar 'Bluecrop' were screened for unique Simple Sequence Repeat (SSR)-containing sequences. Our goal was to develop a set of robust SSR markers for use in managing the National Clonal...
Reed Canarygrass (RCG) is an aggressive invader in aquatic ecosystems that swiftly modifies the environment and decreases biodiversity. A physical model was used to investigate the mechanisms by which RCG spreads. Experimental trials were implemented to examine feedbacks between a mid-channel patch of the flexible RCG, hydraulics, and bedform evolution...
Two studies used DNA markers to assess their usefulness in germplasm identification and evaluation of genetic stability in four cryopreserved Rubus accessions that were stored for over 10 years in liquid nitrogen. In the first study, 12 robust Simple Sequence Repeat (SSR) markers were developed from two microsatellite-enriched libraries of...
The objectives of this project were to determine the effects of harvest maturity, packaging, and storage conditions on subsequent fruit quality of Actinidia arguta ‘Ananasnaya’ and to investigate the use of edible coatings to improve fruit quality and storage life. The influences of harvest maturity (6.0, 8.7, 9.1, and 15.1%...
The Elliott State Forest, located in the Coast Range of Oregon, is currently revising their Habitat Conservation Plan (HCP). Many of the constraints in the HCP are spatial, requiring identification of specific parcels in order to limit activity along habitat reserves, limit harvest opening size, and to coordinate activities within...