Investigations of 16S rRNA gene sequences hallmark modern microbiology. These sequences provide culture-independent insight into the abundance and distribution of microbiota and serve as a principle resource through which microbial community diversity is measured. Consequently, researchers rely on 16S gene sequences to test hypotheses rooted in ecology, evolution, and disease....
A transaminase (aminotransferase, EC 2. 6. 1) was extracted and
purified approximately 148-fold from shoot tips of pea (Pisum sativum
L. cv. Alaska) seedlings and some properties of the enzyme were
determined. The purification included acetone precipitation followed
by column chromatography on hydroxylapatite.
With a-ketoglutarate as co-substrate, the acetone precipitated...
Excessive nitrates in drinking water pose a human health threat, especially to infants. Methemoglobinemia, or blue-baby syndrome, is a potentially fatal condition that inhibits the ability of red blood cells to bind and transport oxygen. Nitrates/nitrites have also been linked to such conditions as cancer, birth defects, and behavioral and...
Livestock grazing is the prominent land use in Wyoming big sagebrush (Artemisia tridentata ssp. wyomingensis [Beetle & A. Young] S.L. Welsh) steppe and has been present since the late 1800’s. There have been calls to remove livestock grazing from rangelands as historic grazing practices resulted in the degradation of some...
Effective and sustainable fisheries management not only depends on identifying and defining stocks (fundamental unit of management), but also on having knowledge of factors influencing the abundance, distribution and connectivity of stocks. Population structure of Pacific cod (Gadus macrocephalus) in the southeastern Bering Sea (EBS) remains unresolved although genetic data...
Pacific cod (Gadus macrocephalus) is an important species, both economically and ecologically in the Bering Sea and Aleutian Islands (BSAI). However, little is known about its spawning dynamics. To address this knowledge gap, I developed a gross anatomical maturity key for Pacific cod to assess temporal and spatial patterns of...
Microbial communities in Arctic coastal lagoons drive biogeochemical cycles at the terrestrial-marine interface and help to determine the fate and form of resources like nitrogen (N) and carbon (C) as they are delivered to the Arctic Ocean. Though rising rates of primary production in the Arctic Ocean are well-characterized, the...
Broad-scale estimates of above ground forest biomass (AGB) are typically produced by applying individual-tree equations to inventory data consisting of measurements from probabilistically or purposively selected trees. The associated uncertainty for these estimates depends primarily on three sources of error that interact and propagate: (1) measurement error, the quality of...
Arbuscular mycorrhizal fungi (AMF) may exert profound influences on ecosystem resilience and invasion resistance in western North American sagebrush steppe and other arid rangeland plant communities. Maintenance of plant community structure through ecological feedbacks such as facilitation of nutrient cycling and uptake by host plants, physical and chemical contributions to...
Heavy timber framing relies primarily on bracing to withstand lateral loads due to earthquakes and wind events. Bracing configurations in heavy timber framed buildings vary widely and include cross bracing, knee bracing, and other geometries. Many heavy timber frames constructed during colonial American times are still standing, exceeding the expected...