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Filtering by: Committee Member Ho, P. Shing Degree Name Doctor of Philosophy (Ph.D.) Subject Chromatin

Effects of nucleosomes on transcription by polymerase I in a reconstituted system

Creator:

Georgel, Philippe, 1961-

Abstract:

The aim of this study was to gain more information about the interactions between DNA and the histone octamer during the process of transcription. This work used a pUC8 plasmid derivative that contained the core promoter region of the RNA polymerase I of Acanthamoeba castellanii, placed upstream of four repeats...

Resource Type:

Dissertation

Full Text:

his support. All the members of my committee, professor Pui Shing Ho, professor Henry Schaup
Towards the understanding of the function of the histone "tails" with respect to the structure, stability, and function of chromatin

Creator:

Dong, Feng

Abstract:

By using immobilized trypsin, I have been able to prepare well-defined, stable trypsinized nucleosomes. The difficulties of lacking of control in the extent of trypsinization, which were encountered in previous studies with the use of free trypsin, have been eliminated. The nucleosomes and oligonucleosomes prepared by immobilized trypsin are suitable...

Resource Type:

Dissertation

Full Text:

graduate committee members, Dr. George Bailey, Dr. Shing Ho, Dr. Christopher Mathews, and Dr. John Morris