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Filtering by: Creator Lee, Tae Hee Remove constraint Creator: Lee, Tae Hee File Format application/pdf Remove constraint File Format: application/pdf Subject Bacterial genetics Remove constraint Subject: Bacterial genetics

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  • Developing a system of mutagenesis in Francisella tularensis LVS

    Creator:
    Flax, Lindsay A.
    Abstract:
    Francisella tularensis is a gram-negative facultative intracellular coccobacillus that primarily infects macrophages. The causative agent of tularemia, this bacterium is considered among the most infectious organisms known, requiring fewer than ten organisms to cause disease. Although ubiquitous in nature, transmission to humans is rare but can occur via insect bites,...
    Resource Type:
    Masters Thesis
    Full Text:
    resolved in 0.7% 16 (wt/vol) agarose gel made in Tris base, Acetic acid, EDTA (TAE). Gels were

  • Identification of an aerotaxis transducer in Vibrio cholerae

    Creator:
    Boin, Markus A.
    Abstract:
    The ability to move towards favorable environmental conditions, called chemotaxis, is common among motile bacteria. In particular aerotaxis has been extensively studied in Escherichia coli. Three putative aer gene homologs were identified in the V. cholerae genome designated VCAer-1 (VC0512) VCAer-2 (VCA0658), and VCAer-3 (VCA0988). Deletion analyses indicated that only...
    Resource Type:
    Masters Thesis
    Full Text:
    PCR products were run on a 1% agarose gel in 1X TAE buffer at 100V for 40 minutes. The gel was