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Pseudoperonospora cubensis and P. humuli detection using species-specific probes and high definition melt curve analysis

Creator:

Summers, Carly F., Adair, Nanci L., Gent, David H., McGrath, Margaret T., and Smart, Christine D.

Abstract:

Real-time PCR assays using locked nucleic acid (LNA) probes and high resolution melt (HRM) analysis were developed for molecular differentiation of Pseudoperonospora cubensis and P. humuli, causal agents of cucurbit and hop downy mildew, respectively. The assays were based on a previously identified single nucleotide polymorphism (SNP) in the cytochrome...

Resource Type:

Article

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: 34 1 4 D ec em be r 20 15 et ceux basés sur l’analyse des HRM nouvellement conçus
Tertiary geologic framework, neotectonics, and petroleum potential of the Oregon-Washington Continental Margin

Creator:

Snavely, P. D., Jr. and Geological Survey (U.S.)

Abstract:

The Tertiary geologic evolution of the Oregon and Washington continental margin was molded by episodic periods of convergence between the Pacific oceanic plates and the North American plate. This margin is the site of a deep basin that is floored by Paleocene to lower Eocene oceanic crust and contains more...

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Part of Book

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. Snavely, Jr. U.S. Geological Survey Menlo Park, California A B S T R A C T The Tertiary geologic