PCR amplification, restriction fragment length polymorphism, and phylogenetic analysis of oxygenase genes
were used for the characterization of in situ methane- and ammonia-oxidizing bacteria from free-living and
attached communities in the Eastern Snake River Plain aquifer. The following three methane monooxygenase
(MMO) PCR primer sets were used: A189-A682, which amplifies...
Ammonia (NH₃)-oxidizing bacteria (AOB) and thaumarchaea (AOA) co-occupy most soils, yet no short-term growth-independent method exists to determine their relative contributions to nitrification in situ. Microbial monooxygenases differ in their vulnerability to inactivation by aliphatic n-alkynes, and we found that NH₃ oxidation by the marine thaumarchaeon Nitrosopumilus maritimus was unaffected...
The myxozoan parasite Ceratomyxa shasta is a significant pathogen of juvenile salmonids in the Pacific Northwest of North America and is limiting recovery of Chinook (Oncorhynchus tshawytscha) and coho (O. kisutch) salmon populations in the Klamath River. We conducted a 5-year monitoring program that comprised concurrent sentinel fish exposures and...
The decomposition of marine plankton in two-chamber, seawater-filled microbial fuel cells (MFCs) has been investigated and related to resulting chemical changes, electrode potentials, current efficiencies, and microbial diversity. Six experiments were run at various discharge potentials, and a seventh served as an open-circuit control. The plankton consisted of a mixture...
The bacteria colonizing geologic core sections (attached) were contrasted with those found suspended in the groundwater (unattached) by examining the microbiology of 16 depth-paired core and groundwater samples using a suite of culture-independent and culture-dependent analyses. One hundred twenty-two meters was continuously cored from a buried chalcopyrite ore hosted in...
Genomic libraries derived from environmental DNA (metagenomic libraries) are useful for characterizing
uncultured microorganisms. However, conventional library-screening techniques permit characterization of
relatively few environmental clones. Here we describe a novel approach for characterization of a metagenomic
library by hybridizing the library with DNA from a set of groundwater isolates, reference...
A previous study showed that ammonia oxidation by the Thaumarchaeota Nitrosopumilus maritimus (group 1.1a) was resistant
to concentrations of the C₈ 1-alkyne, octyne, which completely inhibits activity by ammonia-oxidizing bacteria. In this study, the
inhibitory effects of octyne and other C₂ to C₁₀ 1-alkynes were evaluated on the nitrite production...