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Optimization studies in preparative chromatography of biomolecules

Creator:

Ramanan, Sundar

Abstract:

Optimization of preparative nonlinear chromatography was carried out for the first time for a biomolecule mixture. Conventional wisdom on optimization, which roots from analytical chromatography, dictates optimizing resolution in an analytical column and obtaining similar separation in a large column for isolation. Such a method of optimization significantly under uses...

Resource Type:

Dissertation

Full Text:

biomolecules AN ABSTRACT OF THE DISSERTATION OF Sundar Ramanan for the degree of Doctor of Philosophy in
Biomass productivity enhancement of Laminaria saccharina cultures in a stirred-tank bioreactor by batch and fed-batch nutrient delivery

Creator:

Ramanan, Sundar

Resource Type:

Masters Thesis

Full Text:

OF Sundar Ramanan for the degree of Master of Science in Chemical Engineering presented on December
Preparative purification of chemotactic peptides by gradient elution in reversed-phase chromatography

Creator:

Kim, Billy

Abstract:

Gradient elution chromatography is frequently used for the preparative separation of peptides and proteins. Separations at high loadings are often avoided because peaks become asymmetrical and saturate the detector. However, non-linear interactions which become important at high loadings may actually improve the separation with greater concentrations of the product being...

Resource Type:

Masters Thesis

Full Text:

, Myriam Kucza, Quitterie Motte, Adil Nazir, Nong Podhipleux, Sundar Ramanan, and Jun Wang. I am