Frankia from root nodules of nine different species of Ceanothus were
characterized. DNA was amplified directly from nodular material using the polymerase
chain reaction (PCR). The amplified region includes the 3' end of the 16S rRNA gene,
the intergenic spacer (IGS), and a large portion of the 23S rRNA gene....
Nitrification and denitrification are major biological processes transforming nitrogen (N) in soils to plant available N, highly leachable nitrate (NO₃⁻) and gaseous N oxides. Although many studies in the past have studied N cycling communities, the effect of increased N inputs on ammonia-oxidizer and denitrifier population dynamics is still under...
Few studies have directly compared denitrifying community composition and activities in soils by coupling molecular-genetic techniques and traditional measures of denitnfication. I investigated communities of denitrifying bacteria from adjacent meadow and forest soils in the Cascade Mountains, Oregon. A key gene in the denitrification pathway, N₂O reductase (nosZ), served as...
Soil nitrogen exists largely as organic matter, including plant liter, dead animal matter, and microbial necromass. About 90% of soil organic nitrogen is proteinaceous material that is too large for plants and microorganisms to assimilate directly. Protein depolymerization therefore plays a critical role in mobilizing this organic source of nitrogen,...
Forests are one of the largest repositories of terrestrial C. Understanding factors that drive organic matter transformations and nutrient efflux from these systems is therefore highly important. Temperate forests are of particular significance due to the large fraction of C that is stored below ground in the soil. Characterizing nutrient...
My research has focused on understanding N and C cycling in soils at small scales. Isotope dilution methods commonly used to estimate gross rates of soil chemical transformations assume homogeneous distribution of label. I explored the effects of diffusion limitations on isotope dilution experiments in soil aggregates using spherical diffusion-reaction...
Algae have shown great potential as a source for renewable fuels. However, current production schemes have not been able to prove a sustainable energy return on investment due in part to the high costs of nutrient addition and the energy required for drying the biomass. Integrated algae-dairy production systems have...