Anthropogenic induced climate change is predicted to alter distribution of existing plant populations. As plants migrate over space and time, populations often fragment and contract, affecting basic elements of population dynamics (e.g., population size, gene flow, genetic diversity, etc.). Little is known, however, how these impacts on plant species will...
Rhizopogon salebrosus is an abundant ectomycorrhizal fungus common throughout Western North America that forms mutualistic partnerships with Pinus species. Studied for its systematics, use in sapling nurseries, post-fire seedling establishment, and unique evolutionary history, R. salebrosus holds many secrets within its genome. This thesis aims to characterize the mating system,...
Since the discovery of penicillin in 1928, the fungal kingdom has remained a fertile source for the discovery of new, biologically active, natural products. Further, as natural products continue to be discovered from fungi, a deeper understanding of the critical role of specialized metabolism in fungal ecology is afforded. The...
Bacterial endophytes have the potential to confer benefits to Douglas-fir (Pseudotsuga menziesii
), for instance drought tolerance. Bacterial endophytes originating from seeds are
especially important. This is because seed endophytes are more likely to be the first endophytes
to get established within the young host plant. The goals of...
Bacterial endophytes have the potential to confer benefits to Douglas-fir ( Pseudotsuga menziesii ), for instance drought tolerance. Bacterial endophytes originating from seeds are especially important. This is because seed endophytes are more likely to be the first endophytes to get established within the young host plant. The goals of...
Seeds are an essential component of plant life histories, and seed endophytes have the potential to influence germination, seedling establishment and development. That said, seed endophytes are a relatively new area of study, both in the factors that influence which taxa are present and how these microbes alter plant function....
An increased understanding of secondary metabolism in fungi is important for both biological and societal reasons. The ascomycete genus, Tolypocladium, is an ideal system to explore secondary metabolism characterization, ecology, evolution and regulation. The genus produces and has the genomic potential to produce a wide array of metabolites. Paired with...
Whitebark pine (Pinus albicaulis) is an iconic North American high-elevation tree species currently threatened by climate change, mountain pine beetle, and white pine blister rust (WPBR), a lethal disease caused by the non-native fungal pathogen Cronartium ribicola. In collaboration with the USDA Forest Service Dorena Genetic Resource Center, germplasm was...
In this dissertation, I present the results from three studies: (1) a phylogeny of Tenebrionoidea (Coleoptera) inferred from a seven-gene dataset derived from PCR and Sanger sequencing; (2) a phylogeny of Tenebrionidae, the largest family in Tenebrionoidea, inferred from a four-gene dataset also derived from PCR and Sanger sequencing; (3)...
Research on the population genetics of microbial organisms requires the use of specialized analyses designed for clonal organisms to avoid violating the assumptions of traditional population genetic models. The tools necessary for performing these analyses existed as a set of unrelated software with non-overlapping capabilities and did not cover all...
Rhizopogon vesiculosus is a common ectomycorrhizal (EM) symbiont of Pseudotusga menziesii (Douglas-fir) in the coast range of the Pacific Northwest. The species has been studied for its systematics, genet size, population structure, and competitive ability in several field and experimental studies. This thesis seeks to provide a more thorough characterization...
Many obligate symbiotic fungi are difficult to maintain in culture, and there is a growing need for alternative approaches to obtaining tissue and subsequent genomic assemblies from such species. In this study, the genome of Elaphomyces granulatus was sequenced from sporocarp tissue. The genome assembly remains on many contigs, but...
Fungi in the genus Ascosphaera (Ascomycota: Eurotiomycetes: Ascosphaerales) cause chalkbrood disease in larvae of bees. Here, we report the first-ever detection of the fungus in adult bumble bees that were raised in captivity for studies on colony development. Wild queens of Bombus griseocollis, B. nevadensis and B. vosnesenskii were collected...
Rhizopogon is a large genus of ectomycorrhizal (EM) fungi that grows in association with host trees of the family Pinaceae. We have conducted a series of studies investigating the ecology and evolutionary biology of the EM symbiosis shared between R. subgenus Villosuli and trees of the genus Pseudotsuga. Two members...
Fungi in the genus Tolypocladium are diverse in their host associations, but the predominant ecologies include parasites of the ectomycorrhizal genus Elaphomyces and pathogens of insects. The aim of this dissertation research is to examine the evolution of these fungi and their host associations. To accomplish this several lines of...
Bumble bees (Hymenoptera: Apidae) are important native pollinators in wild and agricultural systems, and are one of the few groups of native bees commercially bred for use in the pollination of a range of crops. In recent years, declines in bumble bees have been reported globally. One factor implicated in...
Rhizopogon subgenus Villosuli are the only
members of the genus known to form an ectomycorrhizal
relationship exclusively with Pseudotsuga. The
specificity of this host relationship is unusual in that
Rhizopogon is broadly associated with several tree
genera within the Pinaceae and relationships with a
host genus are typically distributed across...
Photobiological hydrogen production -- the use of photosynthesis to split water into hydrogen and oxygen -- presents an opportunity for efficient and large-scale solar biofuel development. One of the major challenges in this area is competition for reductant between the hydrogen-evolving hydrogenase enzyme and other metabolic pathways. In this work...
Using spatial autocorrelation analysis, we
examined the within-population genetic structure of
Rhizopogon vinicolor and R. vesiculosus, two hypogeous
ectomycorrhizal (EM) species that are sympatric
sister taxa known to differ in their clonal structure.
We collected 121 sporocarps and 482 tuberculate EM
of both species from a 20 ha forest stand...
Lichens play many important roles in subarctic terrestrial ecosystems by fixing nitrogen, colonizing rock and gravel, stabilizing otherwise bare soil, adding significantly to vegetation biodiversity and serving as the primary food for caribou in the winter. In these chapters, I analyzed lichen community and ecological trait structure along environmental gradients,...