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Filtering by: Subject DNA-protein interactions Remove constraint Subject: DNA-protein interactions Language English [eng] Remove constraint Language: English [eng]

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  • Binding and assembly of H5 (and the globular domain of H5) onto DNA

    Creator:
    Carter, George John
    Abstract:
    In order to better characterize linker histone interactions with DNA, avian erythrocyte-specific linker H5 and the trypsin-resistant globular domain of H5 (GH5) were used in DNA binding studies. To begin, H5 displayed a considerably higher binding affinity for DNA than the isolated globular domain (GH5), supporting the importance of the...
    Resource Type:
    Dissertation
    Full Text:
    protein-DNA contact. Linker histones also induce DNA-protein aggregation in a process that was found to

  • The mechanism of interaction of the linker histone with DNA and nucleosomes

    Creator:
    Ellen, Thomas Patrick
    Abstract:
    This dissertation examines the interaction of the linker histone with DNA and with nucleosomes. The first goal of the project was to characterize the interaction of the linker histone with DNA. Three factors previously reported to influence the linker histone's interaction with DNA were examined: ratio of linker histone to...
    Resource Type:
    Dissertation
    Full Text:
    Mechanism of Interaction of the Linker Histone with DNA and Nucleosomes. Abstract approved: This

  • Mass spectrometric analysis of UV-crosslinked protein-nucleic acid complexes

    Creator:
    Doneanu, Catalin E.
    Abstract:
    The DNA-binding domains of E. coli uracil-DNA glycosylase (Ung) and human replication protein A (hRPA) were studied using a general protocol developed in our laboratory for probing protein-DNA interactions. The procedure involves purification and mass spectrometric analysis of the nucleopeptide-products of a tryptically digested UV-crosslinked protein-nucleic acid complex. In the...
    Resource Type:
    Dissertation
    Full Text:
    . INTRODUCTION 1.1. ANALYSIS OF PROTEIN-DNA INTERACTIONS The study of the interaction of proteins, whether they

  • Biochemical characterization and mutational analysis of human uracil-DNA glycosylase

    Creator:
    Chen, Cheng-Yao
    Abstract:
    PCR-based codon-specific random mutagenesis and site-specific mutagenesis were performed to construct a library of 18 amino acid changes at Arg276 in the conserved leucine-loop of the core catalytic domain of human uracil-DNA glycosylase (UNG). Each Arg276 mutant was then overproduced in E. coli cells and purified to apparent homogeneity by...
    Resource Type:
    Dissertation
    Full Text:
    R276 mutant proteins formed a stable complex with the uracil-DNA glycosylase inhibitor protein (Ugi

  • Host kinases involved in DNA precursor biosynthesis during bacteriophage T4 infection

    Creator:
    Bernard, Mark Aguirre
    Abstract:
    Although the Escherichia coli host has almost all of the enzymes necessary to synthesize nucleotides needed for bacteriophage T4 DNA replication, phage genes expressed early in infection encode enzymes for de novo DNA precursor biosynthesis and salvage from degraded host DNA. Eight early enzymes and two host enzymes comprise the...
    Resource Type:
    Dissertation
    Full Text:
    interaction involving T4 DNA polymerase (gene 43 protein) and T4 ss-DNA binding protein (gene 32 protein) 101

  • Characterization of the Escherichia coli uracil-DNA glycosylase- inhibitor protein interaction

    Creator:
    Bennett, Samuel E.
    Resource Type:
    Dissertation
    Full Text:
    of the Escherichia coli Uracil-DNA Glycosylase Inhibitor Protein Interaction. Abstract approved

  • Escherichia coli uracil-DNA glycosylase : DNA binding, catalysis, and mechanism of action

    Creator:
    Shroyer, Mary Jane N.
    Resource Type:
    Masters Thesis
    Full Text:
    inhibitor (Ugi) protein. Using UV-catalyzed protein/DNA crosslinking to measure DNA binding affinity, the

  • Organization of the T4 dNTP synthetase complex at DNA replication sites

    Creator:
    Kim, JuHyun
    Abstract:
    With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and...
    Resource Type:
    Dissertation
    Full Text:
    , the single-stranded DNA binding protein encoded by gene 32, plays a role in recruiting enzymes of

  • The asymmetric binding of Actinomycin D to DNA hexamers

    Creator:
    Ivancic, Monika
    Abstract:
    The solution structure determination of DNA molecules has been an important part of structural biology. NMR solution structures are a complement to structures solved via X-ray crystallography; the two methods are the only ways of obtaining three dimensional coordinates of macromolecules. Because of the nature of the molecule, the solution...
    Resource Type:
    Dissertation
    Full Text:
    transcribed into RNA. RNA then commuricates the genetic information to ribosomes for protein synthesis. DNA

  • Protein Disorder in the Evolution of Dynein Regulation

    Creator:
    Jie, Jing
    Abstract:
    Intrinsically disordered proteins (IDP) are a class of proteins that lack a three-dimensional structure and their prevalence and diverse functions in the cell have only been discovered relatively recently. The intermediate chain (IC) subunit of the microtubule motor protein complex dynein contains an N-terminal disordered region, N-IC, which is central...
    Resource Type:
    Dissertation
    Full Text:
    , and the DNA damage repair protein Rad50 (Mark et al., 6 2005; Uversky et al., 2008