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  • Activation, incorporation and modification of capreomycidine during viomycin biosynthesis

    Creator:
    Fei, Xiaobo
    Abstract:
    Molecular genetic and enzymological techniques have been employed to study antibiotic biosynthesis. The nonproteinogenic amino acid capreomycidine is the signature residue found in the tuberactinomycin family of antitubercular peptide antibiotics and an important element of the pharmacophore. Recombinant VioG, a single module peptide synthetase from the viomycin gene cluster cloned...
    Resource Type:
    Masters Thesis
    Full Text:
    . Maier, and Dr. Rockey for their guidance and assistance throughout my graduate studies. I would like

  • Some potential mechanisms for finely-tuned regulation of phospholipase C-β isozymes : studies of dimerization and phosphatidylinositol 3,4,5-trisphosphate activation

    Creator:
    Zhang, Yong
    Abstract:
    Phospholipase C-β (PLC-β) isozymes are key effectors in G protein-coupled signaling pathways. Prior research suggested that some isoforms of PLC-β may exist and function as dimers, but little is known about dimerization of PLC-β. Data from coimmunoprecipitation assays of differentially-tagged PLC-β constructs and sizeexclusion chromatography of native PLC-β support homodimerization...
    Resource Type:
    Dissertation
    Full Text:
    . Paul Murtaugh, Dr. Claudia Maier and Dr. Lan Xue for their invaluable support and feedback during

  • Fabrication and application of polymer based microfluidic devices

    Creator:
    Koesdjojo, Myra T.
    Abstract:
    The concept of reducing laboratory operations in scale such that they fit on a microfluidic chip has been met with great enthusiasm. Lab-on-a-chip devices promise to be cost effective to operate due to reduced reagent consumption, have the potential to offer shorter analysis times due to their short path lengths,...
    Resource Type:
    Dissertation
    Full Text:
    to Dr. Chih-hung Chang, Dr. Claudia Maier, Dr. David Hackleman, and Dr. Michael Penner, for their

  • Determination of ethyl glucuronide and ethyl sulfate as human biomarkers of alcohol exposure in urine by liquid chromatography/electrospray tandem mass spectrometry with large volume injection

    Creator:
    Hu, Yan
    Abstract:
    This research was undertaken to develop a sensitive, selective, and rapid analytical method to measure ethyl glucuronide (EtG) and ethyl sulfate (EtS) in urine as urinary biomarkers of alcohol exposure. Large-volume injection (LVI) coupled with liquid chromatography / electrospray tandem mass spectrometry was used to eliminate the need for either...
    Resource Type:
    Masters Thesis
    Full Text:
    . Claudia Maier, and Dr. Fredrick Prahl, for their advice on the dissertation content throughout the

  • Suppression of Met signaling by the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG)

    Creator:
    Larsen, Christine A.
    Abstract:
    Met is a prognostic indicator of colorectal cancer patient survival. Therapies that target Met may therefore have beneficial outcomes in the clinic. Recently, EGCG was reported to suppress Met activation, although the mechanisms were not elucidated. HCT116 and HT29 human colon cancer cells were used to examine the relationships between...
    Resource Type:
    Dissertation
    Full Text:
    . David Williams and Dr. Claudia Maier. Thank you for all of your advice and suggestions. You played

  • Biophysical analyses of peroxiredoxins and a partner reductase

    Creator:
    Hall, Andrea Renée
    Abstract:
    Peroxiredoxins (Prxs) are dominant peroxide-reducing enzymes with two important roles: they protect all organisms from oxidative damage induced by peroxides, and in eukaryotes, they participate in hydrogen peroxide signaling pathways. This dissertation presents studies aimed at the biophysical characterization of select Prxs and a Prx reductase to elucidate their structure-function...
    Resource Type:
    Dissertation
    Full Text:
    Trempy, Claudia Maier and Martin Schuster for your interest in my development as a scientist. I

  • Bacterial hydrophobicity : assessment techniques, applications and extension to colloids

    Creator:
    Saini, Gaurav
    Abstract:
    This research details laboratory assessment of bacterial hydrophobicity, variations in bacterial adhesion properties with growth and extended starvation as well as extension of this assay to colloidal hydrophobicity measurement. Hydrophobicity is an integral component of surface interactions and has been studied extensively for its role in a number of engineering...
    Resource Type:
    Dissertation
    Full Text:
    of my doctoral committee- Profs. Jeff Nason, Jack Istok, and Claudia Maier for their time. I would

  • Development of materials and devices for separation of polyamido[a]mine dendrimers

    Creator:
    Tennico, Yolanda H.
    Abstract:
    Over the last decade, the use of highly branched macromolecules, called dendrimers, is emerging. Polyamidoamine (PAMAM) dendrimers, in particular, have attracted high interest due to their unique properties, such as well-defined size, shape, and solubility in water. Our group, as part of an interdisciplinary team, is currently working on developing...
    Resource Type:
    Masters Thesis

  • Natural products studies of the marine cyanobacteria Lyngbya semiplena and Lyngbya majuscula

    Creator:
    Han, Bingnan
    Abstract:
    This thesis details my investigations of marine cyanobacterial natural products that resulted in the discovery of eighteen new secondary metabolites. Isolation and characterization of these unique molecules were carried out using different chromatographic techniques and by careful analyses of 1D and 2D NMR data, respectively. Preliminary bioassays of the crude...
    Resource Type:
    Dissertation
    Full Text:
    . George Constantine, Dr. Michael Schimerlik, and Dr. Claudia Maier for their support and academic

  • Monolithic sorbents for microscale separations

    Creator:
    Doneanu, Angela
    Abstract:
    Over the last decade, the miniaturization of analytical systems has become an increasingly important and interesting research area. Miniaturized systems offer many advantages, including reduced reagent and sample consumption, shorter analysis times, portability and disposability. This dissertation describes novel approaches in this direction, focusing on two areas: the miniaturization of...
    Resource Type:
    Dissertation
    Full Text:
    Ingle, Dr. Michael Lerner, Dr. Claudia Maier and Dr. Gregory Rorrer for offering the academic guidance