Lignocellulosic biomass has potential as a renewable resource for global energy and bioproducts production. Biomass consists of three main polymers, cellulose, hemicellulose, and lignin. Cellulose is a homopolymer of glucose, while hemicellulose and lignin are heteropolymers. Lignin is primarily made of syringyl (S), guiacayl (G) and p-hydroxyphenyl (H) units. Pretreatment...
The economically viable production of value added fuels and chemicals from lignocellulosic feedstocks hinges on our ability to quickly and efficiently transform structural carbon molecules to end products. The sugars in lignocellulosic biomass are held primarily within cellulose and hemicellulose. Cellulose is composed primarily of glucose monomers which are quickly...
Energy production and consumption is a major environmental and economic concern in the world today. Biofuel produced from lignocellulosic biomass is being explored as an alternative energy source to petroleum. Lignocellulosic biomass is comprised of cellulose, hemicellulose, and lignin. To produce biofuels, lignocellulosic biomass must be broken down to expose...
In order to reduce the cost of lignocellulosic ethanol production, a model was previously developed to predict the amount of fermentable sugar released based on parameters concerning both biomass and enzymes. To validate this model, enzymatic hydrolysis must be carried out using individual purified cellulase enzymes. Endo-1,4-β-D-glucanase I (EG I),...
Attributional and consequential life cycle assessments are useful in evaluating the environmental impacts of a process, product, or decision. Both types of life cycle assessments differ however in their scope and their ability to answer specific questions. In order to fully understand and compare the environmental impacts of a sugarcane...
This thesis documents the work carried out investigating the downstream processing of algal biomass for biofuel production. A life cycle assessment was conducted on a hypothetical algal biodiesel process in which it was found drum drying and cell component separation were life cycle intensive processes. A
laboratory experiment was conducted...
The production of fuel ethanol from lignocellulosic biomass has the potential to replace a significant portion of non-renewable transport fuels. Woody feedstocks are composed of cellulose, hemicellulose, and lignin. Glucose, the monomer of cellulose, is readily utilized by wild-type S. cerevisiae, but xylose, which comprises 60% of the sugar in...
Enzymatic hydrolysis is a critical process in the conversion of lignocellulosic biomass into biofuels and biochemicals. Achieving high efficiencies and productivity during the enzymatic hydrolysis of biomass is the key for the commercially viable process. Downstream processing challenges require high product titers which in turn require the use of high...
A major challenge in ethanol production using lignocellulosic feed stock is
inefficient utilization of hemicellulose, which accounts for 30-40% of lignocellulosic
biomass. Xylose, comprising >60% of recoverable sugars from hemicellulose is a
major product of the hemicellulose hydrolysis. Utilization of this carbon source would
significantly increase the ethanol yield from...
Ethanol production from lignocellulosic feedstock has been under intense scrutiny as a transportation fuel due to its potential to address concerns of increasing energy consumption, limited fossil energy resources, climate changes due to greenhouse gas emissions from fossil fuels, and especially use of non-food biomaterials, which address the biggest limitation...