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Filtering by: Creator Simon, Cheyanne M. Remove constraint Creator: Simon, Cheyanne M. License All rights reserved Remove constraint License: All rights reserved Subject Bacterial genetics Remove constraint Subject: Bacterial genetics

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  • Identification of an aerotaxis transducer in Vibrio cholerae

    Creator:
    Boin, Markus A.
    Abstract:
    The ability to move towards favorable environmental conditions, called chemotaxis, is common among motile bacteria. In particular aerotaxis has been extensively studied in Escherichia coli. Three putative aer gene homologs were identified in the V. cholerae genome designated VCAer-1 (VC0512) VCAer-2 (VCA0658), and VCAer-3 (VCA0988). Deletion analyses indicated that only...
    Resource Type:
    Masters Thesis
    Full Text:
    cold 01.M MgCl2. The cells were centrifuged again and the supernatant discarded. Next, the cells were

  • Evolution of a streamlined cooperator in Pseudomonas aeruginosa quorum sensing

    Creator:
    Huie, Jessica L.
    Abstract:
    Social behavior leading to the production of common goods is prone to exploitation. One such behavior in Pseudomonas aeruginosa is quorum sensing (QS), by which the bacteria produce signals to regulate extracellular common goods. Exploitation comes in the form of cheaters which have a mutation in the central quorum sensing...
    Resource Type:
    Masters Thesis
    Full Text:
    essential, it is prone to exploitation, which can come in many forms. In M. xanthus it occurs through

  • Quorum sensing gene regulation in Pseudomonas aeruginosa

    Creator:
    Gupta, Rashmi
    Abstract:
    Pseudomonas aeruginosa is an opportunistic human pathogen that infects immunocompromised individuals such as those suffering from burns or the genetic disorder cystic fibrosis. This organism utilizes a cell-cell communication mechanism known as quorum sensing (QS) to coordinate virulence gene expression and biofilm formation. It has three interconnected QS systems, namely...
    Resource Type:
    Dissertation

  • An investigation of carbon and nitrogen metabolism through a genomic analysis of the genus Nitrobacter

    Creator:
    Starkenburg, Shawn R.
    Abstract:
    The chemolithoautotrophic nitrite oxidizing bacteria (NOB) participate in the biogeochemical cycling of nitrogen by catalyzing and conserving energy from the oxidation of nitrite (NO₂-) to nitrate (NO₃-) via a nitrite oxidoreductase (NXR). The main objective of this work was to comparatively annotate and analyze the genome sequences of Nitrobacter winogradskyi...
    Resource Type:
    Dissertation

  • Molecular and genetic assessment of selected antiporters and methyl-accepting chemotaxis proteins in Vibrio cholerae

    Creator:
    Quinn, Matthew J.
    Abstract:
    The pathogen Vibrio cholerae uses cations as a primary currency of virulence and environmental persistence, using gradients of those cations to move, acquire nutrients, and control virulence gene expression. An understanding of the overlapping roles of bioenergetics and chemotaxis in the virulence and environmental survival of V. cholerae issues from...
    Resource Type:
    Dissertation
    Full Text:
    rotate at over 100,000 rpm (Magariyama, et al., 1994) with a speed of over 60 m per second (Atsumi, et