Search Constraints

Start Over
Filtering by: Creator Smith, Susan M. Remove constraint Creator: Smith, Susan M. License All rights reserved Remove constraint License: All rights reserved Subject Bacterial genetics Remove constraint Subject: Bacterial genetics

Search Results

  • Prokaryotes associated with marine crust

    Creator:
    Mason, Olivia Underwood
    Abstract:
    Oceanic crust covers nearly 70% of the Earth's surface, of which, the upper, sediment layer is estimated to harbor substantial microbial biomass. Marine crust; however, extends several kilometers beyond this surficial layer, and includes the basalt and gabbro layers. In particular, the basalt layer has high permeabilities which allows for...
    Resource Type:
    Dissertation
    Full Text:
    also like to thank my committee members, Drs. Michael S. Rappé, Ricardo M. Letelier, Mario E. Magana

  • Molecular and cellular characterization of the intracellular phenotype of Mycobacterium avium

    Creator:
    Early, Julie V.
    Abstract:
    Mycobacterium avium cause disseminated disease in immunocompromised people such as AIDS patients. Subsequent to crossing the intestinal epithelium, M. avium thrive within vacuoles in macrophages. The bacteria exhibit a different, more invasive, phenotype after being in macrophages compared to M. avium from laboratory conditions. We hypothesized that this intracellular phenotype...
    Resource Type:
    Dissertation
    Full Text:
    immunocompromised people such as AIDS patients. Subsequent to crossing the intestinal epithelium, M. avium

  • Mechanisms for the interaction of environmental mycobacteria with host cells

    Creator:
    Harriff, Melanie J.
    Abstract:
    Environmental mycobacteria are important opportunistic pathogens for many hosts, including humans, cattle, and fish. Two well-studied species are Mycobacterium avium subsp. avium, a significant cause of disseminated bacterial disease in patients with AIDS, and Mycobacterium avium subsp. paratuberculosis, the cause of Johne’s disease in cattle. Many other species that are...
    Resource Type:
    Dissertation
    Full Text:
    and proteins involved in the uptake of M. avium by HEp-2 cells were analyzed by a variety of methods

  • Genomics-enabled exploration of insect toxicity in the Pseudomonas fluorescens group

    Creator:
    Rangel, Lorena I.
    Abstract:
    Pseudomonas is a diverse genus of Gamma proteobacteria that are ubiquitous in the natural environment, including soil, water, plant surfaces, and animals. The Pseudomonas fluorescens group is a diverse collection of seven subgroups and more than 50 named species. This group is known for their production of a variety of...
    Resource Type:
    Masters Thesis
    Full Text:
    injectable lethality to larvae of M. sexta, while four of the strains exhibited no significant

  • Evolution of a streamlined cooperator in Pseudomonas aeruginosa quorum sensing

    Creator:
    Huie, Jessica L.
    Abstract:
    Social behavior leading to the production of common goods is prone to exploitation. One such behavior in Pseudomonas aeruginosa is quorum sensing (QS), by which the bacteria produce signals to regulate extracellular common goods. Exploitation comes in the form of cheaters which have a mutation in the central quorum sensing...
    Resource Type:
    Masters Thesis
    Full Text:
    essential, it is prone to exploitation, which can come in many forms. In M. xanthus it occurs through

  • Analysis of Bacteroidales 16S rRNA gene sequences from a geographically isolated human population

    Creator:
    Petersen, Carolyn A.
    Abstract:
    Standard methods of measuring fecal pollution in water do not distinguish between human and non-human sources. Molecular technology enabled the development of host-specific markers that distinguish fecal sources. Human specific PCR primers, HF183F and HF134F, were designed based on phylogenetic analyses of partial 16S rRNA gene sequences from the Bacteroidales...
    Resource Type:
    Masters Thesis
    Full Text:
    , and 3 washes were done with SEWS-M buffer. Water samples: Bacterial DNA from water samples was

  • Quorum sensing gene regulation in Pseudomonas aeruginosa

    Creator:
    Gupta, Rashmi
    Abstract:
    Pseudomonas aeruginosa is an opportunistic human pathogen that infects immunocompromised individuals such as those suffering from burns or the genetic disorder cystic fibrosis. This organism utilizes a cell-cell communication mechanism known as quorum sensing (QS) to coordinate virulence gene expression and biofilm formation. It has three interconnected QS systems, namely...
    Resource Type:
    Dissertation
    Full Text:
    , Rumbaugh et al., 2000, Smith & Iglewski, 2003). There are two complete acyl-homoserine lactone (acyl

  • Identification of an aerotaxis transducer in Vibrio cholerae

    Creator:
    Boin, Markus A.
    Abstract:
    The ability to move towards favorable environmental conditions, called chemotaxis, is common among motile bacteria. In particular aerotaxis has been extensively studied in Escherichia coli. Three putative aer gene homologs were identified in the V. cholerae genome designated VCAer-1 (VC0512) VCAer-2 (VCA0658), and VCAer-3 (VCA0988). Deletion analyses indicated that only...
    Resource Type:
    Masters Thesis
    Full Text:
    cold 01.M MgCl2. The cells were centrifuged again and the supernatant discarded. Next, the cells were

  • Understanding chlamydial biology through genome sequencing and comparative genomics

    Creator:
    Jeffrey, Brendan
    Abstract:
    The human pathogen Chlamydia trachomatis displays different phenotypes between serovars both in vivo and in vitro. The study of chlamydial biology has been hampered by the lack of a tractable genetic system. Here, chlamydial isolates with known phenotypes as well as strains generated though recombination were fully sequenced to address...
    Resource Type:
    Dissertation
    Full Text:
    Chapter 1: Brendan M. Jeffrey and Dan D. Rockey wrote the manuscript. Chapter 2: Robert J. Suchland

  • Characterization of the genomic islands from tetracycline-resistant Chlamydia suis

    Creator:
    Dugan, Jae
    Abstract:
    Tetracycline (Tet)-resistant strains of Chlamydia suis were isolated from swine farms in the Midwest. The isolation of the resistant strains was significant because Tet is an antibiotic used to treat infections by veterinarians and doctors. One of the tetracycline (Tet)-resistant strains, R19, was able to survive in Tet up to...
    Resource Type:
    Dissertation