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Filtering by: Creator Aldwin, Carolyn M. License All rights reserved Subject Cellulase -- Absorption and adsorption

Assaying the activities of Thermomonospora fusca E₅ and Trichoderma reesei CBHI cellulases bound to polystyrene

Creator:

Kongruang, Sasithorn

Abstract:

In this study the enzymatic activity of adsorbed Thermomonospora fusca E₅ and Trichoderma reesei CBHI cellulases were investigated using fluorescence techniques. In particular, cellulases were allowed to contact hydrophobic polystyrene surfaces under conditions of different solution concentrations, and adsorption times. Each of these variables is known to have a potential...

Resource Type:

Masters Thesis

Full Text:

Carolyn S. Baker for their suggestions about the cellulase experiments. Also, thank you Nong, Patty, and
Adsorption of Trichoderma reesei CBHI and Thermomonospora fusca E₅ cellulases on model solid surfaces

Creator:

Baker, Carolyn S.

Abstract:

In this research, the interfacial behavior of Trichoderma reesei CBHI and Thermomonospora fusca E₅ cellulases were studied at synthetic surfaces. For this purpose, colloidal silica and polystyrene particles were used to prepare cellulase-particle suspensions that were analyzed by several solution-phase techniques. These included circular dichroism spectroscopy, size exclusion chromatography and...

Resource Type:

Masters Thesis

Full Text:

fusca E5 cellulases on model solid surfaces AN ABSTRACT OF THE THESIS OF Carolyn S. Baker for the